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Updated: Jan 20, 2026

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
Stable Isotope-Resolved Metabolomics by NMR.
Penghui Lin1, Andrew N Lane2,3, Teresa W-M Fan1,4
1Center for Environmental and Systems Biochemistry, University of Kentucky, Lexington, KY, USA.
Stable isotopes and Nuclear Magnetic Resonance (NMR) spectroscopy allow precise tracking of atoms through metabolic pathways. This chapter details NMR methods for analyzing positional isotopomer distribution in metabolic tracer studies.
Area of Science:
- Metabolomics
- Biochemistry
- Analytical Chemistry
Background:
- Stable isotopes are crucial for tracing metabolic pathways.
- Nuclear Magnetic Resonance (NMR) spectroscopy offers unique analytical capabilities.
- NMR enables atom position-resolved isotope analysis and isotope editing for metabolic studies.
Purpose of the Study:
- To describe NMR-based methods for analyzing positional isotopomer distribution.
- To highlight the application of NMR in metabolic tracer studies.
Main Methods:
- Utilizing stable isotopes as metabolic tracers.
- Applying Nuclear Magnetic Resonance (NMR) spectroscopy.
- Performing atom position-resolved isotope analysis.
- Employing isotope editing techniques.
Main Results:
- Demonstration of NMR's capability for detailed metabolic tracing.
- Analysis of positional isotopomer distribution in metabolic products.
- In situ and in vitro metabolic transformation analysis.
Conclusions:
- NMR spectroscopy is a powerful tool for quantitative metabolic analysis.
- Positional isotopomer distribution analysis using NMR provides insights into metabolic pathways.
- Stable isotope-labeled tracers combined with NMR advance metabolic research.
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