Related Experiment Video

Updated: Jan 20, 2026

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.6K

Analysis of Aggrecanase Activity Using Neoepitope Antibodies

Salvatore Santamaria1, Kazuhiro Yamamoto2

  • 1Centre for Haematology, Imperial College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Abstract

Keywords:
ADAMTSAggrecanAggrecanase activityNeoepitope antibodies

More Related Videos

An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies03:32

An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies

456
Analysis of Histone Antibody Specificity with Peptide Microarrays09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

41.7K

Related Concept Videos

Antibody-Free Assay for RNA Methyltransferase Activity Analysis08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Here, an antibody-free in vitro assay for direct analysis of methyltransferase activity on synthetic or in vitro transcribed RNA is...
7.6K
An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies03:32

An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies

This video demonstrates FcγRIIIa-driven events initiated by therapeutic antibodies in human natural killer cells. The artificial stimulation platform facilitates the exploration of downstream effector functions, encompassing cytoskeletal rearrangement, degranulation, chemokine/cytokine production, and signaling pathways mediated by the FcγRIIIa and Fc portions of antibodies involved in...
456
Analysis of Histone Antibody Specificity with Peptide Microarrays09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.
41.7K
Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies03:03

Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies

This video demonstrates an assay measuring the antibody-mediated activation of T cell effector functions. Adding an influenza virus hemagglutinin (HA)-specific monoclonal antibody to transfected mammalian cells expressing HA results in the formation of immune complexes with HA. Upon introducing engineered T cells expressing Fc receptors and a luciferase reporter, the T cells are activated by the antibody-HA complexes, and this activation is detected by adding a luciferase substrate and...
623
An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα05:54

An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα

This video demonstrates a technique for assessing the efficacy of antibodies against tumor necrosis factor-alpha (TNFα), an inflammatory cytokine, through an in vitro bioanalytical approach. TNFα, when introduced to stressed cells, triggers apoptosis. However, binding to the antibodies confines TNFα, averting apoptosis. The degree of apoptosis is quantified using a bioluminescence...
457
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.4K