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Related Concept Videos

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization06:01

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Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
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micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue...
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Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ...
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Related Experiment Video

Updated: Jan 20, 2026

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

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Expression Analysis by RNAscope™ In Situ Hybridization.

Timothy J Mead1, Suneel S Apte2

  • 1Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

RNA in situ hybridization (ISH) reveals precise spatial and temporal gene expression patterns in matrix biology. This method identifies the cell of origin for matrix molecules, complementing protein localization studies.

Keywords:
Gene expressionIn situRNATissue

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Area of Science:

  • Matrix biology
  • Molecular biology
  • Gene expression analysis

Background:

  • RNA in situ hybridization (ISH) is crucial for understanding gene expression in biological contexts.
  • Immunohistochemistry localizes proteins, but ISH identifies the originating cells.

Purpose of the Study:

  • To detail a protocol for RNA in situ hybridization (ISH) on tissue sections.
  • To highlight the complementary role of ISH alongside immunohistochemistry in matrix biology.

Main Methods:

  • The protocol involves staining tissue sections to visualize gene expression.
  • Focuses on the in situ detection of RNA transcripts.

Main Results:

  • ISH allows for the precise spatial and temporal discrimination of gene expression patterns.
  • Enables identification of the cell type responsible for matrix molecule synthesis.

Conclusions:

  • ISH provides essential information on the life cycle of matrix molecules, such as ADAMTS proteases.
  • This method is vital for a comprehensive understanding of matrix biology and cell-specific gene expression.