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Updated: Jan 20, 2026

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
Published on: December 8, 2023
Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates
Rahel Schnellmann1,2,3, Ruth Chiquet-Ehrismann4,5
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. rschnel3@jhu.edu.
Abstract:
Here we describe the use of a decellularized ECM produced in vitro by BALB/c 3T3 fibroblasts for the identification of ADAMTS substrates. Seeding of ADAMTS protease-producing HEK cells on top of the cell-free ECM followed by analysis of the conditioned medium by liquid chromatography tandem mass spectrometry (LC-MS/MS), allows for screening of ADAMTS substrates without prior purification of full-length protease.
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The Extracellular Matrix

