Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System02:55

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System

671
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T...
671
Genome Engineering of Primary Human B Cells Using CRISPR/Cas908:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

6.9K
Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell...
6.9K
Surface Spreading and Immunostaining of Yeast Chromosomes12:06

Surface Spreading and Immunostaining of Yeast Chromosomes

10.5K
A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread...
10.5K
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells07:56

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

11.8K
Here, we present a protocol to genetically edit CAR-T cells via a CRISPR/Cas9...
11.8K
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas904:22

A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9

541
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
541
CRISPR01:59

CRISPR

57.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

The genetics of the many forms of diversity.

Genetics·2026
Same author

Microbial domestication: Farmhouse brewing preserves a hidden reservoir of yeast diversity.

Current biology : CB·2026
Same author

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.

Nature communications·2026
Same author

Population-scale chemical response revealed by a barcoded yeast collection.

Nature communications·2026
Same author

Association Between Femoral Neck Bone Mineral Density and Osteoporotic Fracture Counts in U.S. Adults: A NHANES 2005-2020 Analysis.

Orthopedic reviews·2026
Same journal

Genetic survey of biomarkers at early and mid-pregnancy identifies pregnancy-specialized immune regulation.

PLoS genetics·2026
Same journal

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program.

PLoS genetics·2026
Same journal

Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

PLoS genetics·2026
Same journal

Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

PLoS genetics·2026
Same journal

Multiple instance fine-mapping: Predicting causal regulatory variants with a deep sequence model.

PLoS genetics·2026
Same journal

Nuclear ubiquitin-conjugating enzyme TrUbc4 and F-box protein TrFwd1-mediated modification of Cre1 in Trichoderma reesei establishes a regulatory mechanism for carbon catabolite repression.

PLoS genetics·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

6.9K

Reshuffling yeast chromosomes with CRISPR/Cas9.

Aubin Fleiss1, Samuel O'Donnell1, Téo Fournier2

  • 1Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France.

Plos Genetics
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a CRISPR/Cas9 method for precise genome engineering, enabling researchers to study chromosomal translocations and their impact on yeast phenotypes. The technique effectively separates structural variations from base substitutions, revealing insights into fitness advantages.

More Related Videos

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
02:55

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System

671
Surface Spreading and Immunostaining of Yeast Chromosomes
12:06

Surface Spreading and Immunostaining of Yeast Chromosomes

Published on: August 9, 2015

10.5K

Related Experiment Videos

Last Updated: Jan 20, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

6.9K
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
02:55

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System

671
Surface Spreading and Immunostaining of Yeast Chromosomes
12:06

Surface Spreading and Immunostaining of Yeast Chromosomes

Published on: August 9, 2015

10.5K

Area of Science:

  • Genetics
  • Molecular Biology
  • Yeast Genetics

Background:

  • Quantifying the fitness impact of chromosomal translocations is challenging due to confounding base substitutions.
  • Understanding how chromosomal architecture influences phenotypes is crucial for biological research.

Purpose of the Study:

  • To develop a novel CRISPR/Cas9-based method for generating markerless and scarless translocations in the yeast genome.
  • To distinguish the phenotypic effects of translocations from those of base substitutions.

Main Methods:

  • CRISPR/Cas9 technology was employed to induce targeted and multiple reciprocal translocations.
  • Chimeric donor DNAs and homologous recombination facilitated trans-chromosomal repair.
  • Repair using endogenous LTR copies enabled multiple translocation generation.

Main Results:

  • The developed method efficiently generates markerless, scarless translocations at base-pair resolution.
  • Recapitulating a specific translocation revealed complex genotype-phenotype relationships regarding sulfite resistance.
  • Multiple translocations induced significant phenotypic diversity and fitness advantages in various environmental conditions.

Conclusions:

  • The novel CRISPR/Cas9 technique effectively untangles the phenotypic impacts of structural variations from base substitutions.
  • Chromosomal architecture reconfiguration alone can confer fitness advantages under stress.
  • This method provides a powerful tool for studying genome structure-phenotype relationships.