Impact of Novel Monoclonal Antibody Therapeutics on Blood Bank Pretransfusion Testing
Related Experiment Video
Updated: Jan 20, 2026

Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
1University of Chicago Medicine, 5841 South Maryland Avenue, AMB S339, MC 3083, Chicago, IL 60637, USA.
Abstract:
Novel monoclonal antibody therapies are increasing in number and clinical significance as their role in oncologic formularies expands. Anti-CD38 and anti-CD47/SIRPα agents commonly interfere with pretransfusion compatibility testing. Anti-CD38 interference is mitigated by dithiothreitol, which disrupts CD38 antigen on reagent red cells; however, this modification limits rule-out of all clinically significant antibodies. Several anti-CD47 agents are in clinical trials and demonstrate wide variability in pretransfusion testing interference. Modifications to pretransfusion testing can limit interference by anti-CD47 agents. Rapid dissemination of knowledge of these monoclonal antibody agents to the broader transfusion medicine community is paramount for continued patient transfusion safety.
Related Concept Videos
12:15Generation of Monoclonal Antibodies Against Natural Products
Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455
2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455
3 Department of Urology, University of Minnesota, Minneapolis, MN 55455
4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455
Polyclonal antibodies are defined as a collection of antibodies directed against...
13:14Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
09:42Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:57Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
09:45Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
