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Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems05:47

Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems

This article describes RUGGED (Retrieval Under Graph-Guided Explainable disease Distinction), which integrates Large Language Model (LLM) inference with Retrieval-Augmented Generation (RAG). It draws evidence from expert-curated biomedical knowledge bases and peer-reviewed biomedical publications to synthesize new knowledge from up-to-date information, identify explainable and actionable predictions, and pinpoint promising directions for hypothesis-driven...
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Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes11:05

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes

The following paper presents a novel FE simulation technique (KBC-FE), which reduces computational cost by performing simulations on a cloud computing environment, through the application of individual modules. Moreover, it establishes a seamless collaborative network between world leading scientists, enabling the integration of cutting edge knowledge modules into FE...
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A Knowledge Graph Approach to Elucidate the Role of Organellar Pathways in Disease via Biomedical Reports07:35

A Knowledge Graph Approach to Elucidate the Role of Organellar Pathways in Disease via Biomedical Reports

A computational protocol, CaseOLAP LIFT, and a use case are presented for investigating mitochondrial proteins and their associations with cardiovascular disease as described in biomedical reports. This protocol can be easily adapted to study user-selected cellular components and...
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New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals09:27

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

CD4+ Regulatory T cells are potent immune-modulators and serve important functions in immune homeostasis. The paucity of these cells in peripheral blood makes functional studies challenging, specifically in the context of HIV-1-infection. We here describe a method to isolate and expand functional CD4+ Tregs from peripheral blood from HIV-1-infected...
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Exploring the Role of Deontic Reasoning and World Knowledge in Wason´s Selection Task06:08

Exploring the Role of Deontic Reasoning and World Knowledge in Wason´s Selection Task

This study aims to analyze how people reason with and about normative rules, deontic reasoning, and the importance of world knowledge in subjects´ inferences. For this purpose, one of the main experimental paradigms in reasoning research was used: Wason´s selection...
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Generation of Natural Killer Cells from Human Expanded Potential Stem Cells06:53

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.
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