Draft Genome Sequence of Nitrosococcus oceani Strain NS58, a Marine Ammonia-Oxidizing Gammaproteobacterium Isolated

Hideo Dohra1, Kentaro Arai2, Hidetoshi Urakawa3

  • 1Instrumental Research Support Office, Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan.

Related Concept Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

The feasibility of whole-genome sequencing (WGS) strategies using benchtop instruments has simplified the genome interrogation of every microbe of public health relevance in a lab setting. A methodological adaptation of the workflow for bacterial WGS is described and a bioinformatics pipeline for analysis is also...
2.8K
Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Microbial sulfate reduction is a process of great importance in environmental biotechnology. The success of the sulfidogenic reactors depends among other factors on the microbial composition of the sludge. Here, we present a protocol to develop sulfidogenic sludge from hydrothermal vents sediments in a UASB reactor for reductive dechlorination...
10.1K
Screening Marine Bacteria for the Isolation of Pollutant-Degrading Strains04:04

Screening Marine Bacteria for the Isolation of Pollutant-Degrading Strains

Source: Villela, H. D. M., et al. Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation. J. Vis. Exp. (2019).This video demonstrates the isolation of pollutant-degrading marine bacteria through serial dilution, colony selection, and growth in EE2-containing medium. By using EE2 as the sole carbon source, the protocol identifies bacterial strains capable of metabolizing this environmental...
158
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

A methodology to isolate high molecular weight and high quality genomic DNA from soil microbial community is described.
26.4K
Novel Sequence Discovery by Subtractive Genomics09:40

Novel Sequence Discovery by Subtractive Genomics

The purpose of this protocol is to use a combination of computational and bench research to find novel sequences that cannot be easily separated from a co-purifying sequence, which may be only partially...
9.1K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human...
23.9K