Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

3.5K
The protocol provides a detailed method of neuronal imaging in brain slice using a tissue clearing method, ScaleSF. The protocol includes brain tissue preparation, tissue clarification, handling of cleared slices and confocal laser scanning microscopy imaging of neuronal structures from mesoscopic to microscopic...
3.5K
A Hydrophobic Tissue Clearing Method for Rat Brain Tissue08:24

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

4.5K
Here we present a hydrophobic tissue clearing method that allows for the viewing of target molecules as part of intact brain structures. This technique has now been validated for F344/N control and HIV-1 transgenic rats of both...
4.5K
Optical Clearing of Plant Tissues for Fluorescence Imaging04:55

Optical Clearing of Plant Tissues for Fluorescence Imaging

6.6K
Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of cleared plant tissues without physical...
6.6K
Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels11:01

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

6.2K
This protocol presents a method for decellularization and subsequent hydrogel formation of murine mammary fat pads following ex vivo...
6.2K
A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample02:08

A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample

510
Source: Kirchner, K.N., et al., A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (2020).This video demonstrates the method of hydrophobic tissue clearing of a rat brain tissue sample. The sample is sequentially dehydrated in methanol, treated with a dichloromethane and methanol mixture to dissolve lipids and pigments, and transferred to dibenzyl ether for complete tissue clearing, enabling detailed imaging without thin...
510
Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue08:52

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue

28.8K
Due to the high lipid content, adipose tissue has been challenging to visualize using traditional histological methods. Adipo-Clear is a tissue clearing technique that allows robust labeling and high-resolution volumetric fluorescent imaging of adipose tissue. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for...
28.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancement in catalytic activity of CotA-laccase from Bacillus pumilus W3 via site-directed mutagenesis.

Journal of bioscience and bioengineering·2019
Same author

Characteristics and evaluation of urban soundscapes worthy of preservation.

Journal of environmental management·2019
Same author

A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.

The Journal of experimental medicine·2019
Same author

[Determination of pesticide residues in wolfberry using QuEChERS-gas chromatography-tandem mass spectrometry].

Se pu = Chinese journal of chromatography·2019
Same author

Amentoflavone induces cell cycle arrest, apoptosis, and autophagy in BV-2 cells.

Frontiers in bioscience (Landmark edition)·2019
Same author

Biosynthesis of Novel Shikonin Glucosides by Enzymatic Glycosylation.

Chemical & pharmaceutical bulletin·2019
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.5K

Study on the tissue clearing process using different agents by Mueller matrix microscope.

Qiaolin Xie1,2,3, Nan Zeng1,3, Yu Huang1,2

  • 1Tsinghua University, Graduate School at Shenzhen, Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, 2279 Lishui Street, Shenzhen 518055, China.

Biomedical Optics Express
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

Mueller matrix microscopy reveals how clearing agents alter tissue optics. Different agents like formamide and sucrose cause opposite phase retardance trends by affecting microstructural features such as collagen and refractive index.

More Related Videos

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue
08:24

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Published on: December 23, 2020

4.5K
Optical Clearing of Plant Tissues for Fluorescence Imaging
04:55

Optical Clearing of Plant Tissues for Fluorescence Imaging

Published on: January 5, 2022

6.6K

Related Experiment Videos

Last Updated: Jan 20, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.5K
A Hydrophobic Tissue Clearing Method for Rat Brain Tissue
08:24

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Published on: December 23, 2020

4.5K
Optical Clearing of Plant Tissues for Fluorescence Imaging
04:55

Optical Clearing of Plant Tissues for Fluorescence Imaging

Published on: January 5, 2022

6.6K

Area of Science:

  • Biophotonics
  • Tissue Engineering
  • Optical Microscopy

Background:

  • In vitro tissue clearing is crucial for biological imaging.
  • Understanding how clearing agents affect tissue microstructure and optical properties is essential.
  • Mueller matrix microscopy offers advanced polarization-based analysis for tissue characterization.

Purpose of the Study:

  • To investigate the in vitro tissue clearing process of mouse dorsal skin using Mueller matrix microscopy.
  • To analyze the impact of different clearing agents (formamide and saturated sucrose) on tissue polarization properties.
  • To elucidate the microstructural and optical changes induced by clearing agents through modeling and simulation.

Main Methods:

  • Monitoring in vitro tissue clearing using Mueller matrix microscopy.
  • Applying Mueller matrix polar decomposition to analyze polarization changes.
  • Developing and utilizing computational models to simulate microstructural and optical changes during clearing.
  • Comparing experimental results with simulations based on various mechanisms (refractive index matching, collagen shrinkage, fiber organization, birefringence).

Main Results:

  • A significant difference in phase retardance trends was observed between formamide and sucrose clearing.
  • Individual mechanisms could not fully explain the observed polarization phenomena.
  • Simulations suggest formamide primarily causes collagen shrinkage and birefringence reduction.
  • Simulations indicate sucrose primarily involves refractive index matching and increased birefringence.
  • Both experimental and simulation data validate Mueller matrix microscopy's utility in studying clearing agent-tissue interactions.

Conclusions:

  • Mueller matrix microscopy effectively characterizes tissue clearing dynamics.
  • Different clearing agents induce distinct microstructural and optical alterations.
  • Formamide and sucrose impact tissue properties differently, leading to varied polarization responses.
  • Combined mechanisms are necessary to accurately model tissue clearing effects.