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Related Concept Videos

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance10:51

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This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally...
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Related Experiment Video

Updated: Jan 20, 2026

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
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Organ-on-e-chip: Three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic

Anna Kalmykov1, Changjin Huang2,3, Jacqueline Bliley1

  • 1Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

Science Advances
|August 31, 2019
PubMed
Summary

New 3D biosensor arrays enable simultaneous, multisite electrophysiology of human cardiac spheroids. This organ-on-e-chip technology advances tissue development and drug discovery for conditions like arrhythmias.

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Last Updated: Jan 20, 2026

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Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Tissue Engineering

Background:

  • Cell-cell communication is crucial for biological systems.
  • Three-dimensional (3D) spheroids mimic in vivo microenvironments for studying cellular communication.
  • Current electrophysiology methods lack direct, simultaneous, multisite investigation of 3D tissues.

Purpose of the Study:

  • To develop and implement 3D self-rolled biosensor arrays (3D-SR-BAs) for interfacing with human cardiac spheroids.
  • To enable direct, multisite, simultaneous electrophysiological recordings in 3D cellular assemblies.
  • To advance the organ-on-an-electronic-chip (organ-on-e-chip) platform for tissue development and drug discovery.

Main Methods:

  • Implementation of 3D self-rolled biosensor arrays (3D-SR-BAs) with active field-effect transistors or passive microelectrodes.
  • Interfacing 3D-SR-BAs with human cardiac spheroids for electrophysiological recordings.
  • Simultaneous calcium imaging alongside field potential recordings.

Main Results:

  • Continuous and stable multiplexed recordings of field potentials from human cardiac spheroids.
  • High sensitivity and spatiotemporal resolution achieved in 3D electrophysiological measurements.
  • Successful integration of electrophysiology and calcium imaging for comprehensive signal transduction analysis.

Conclusions:

  • 3D-SR-BAs provide a novel platform for investigating complex signal transduction in 3D cellular assemblies.
  • This organ-on-e-chip approach supports tissue maturation studies and drug development for diseases like arrhythmias.
  • The technology enables advanced electrophysiological investigation of cardiac spheroids in a physiologically relevant 3D context.