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Related Concept Videos

A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying04:52

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Related Experiment Video

Updated: Jan 20, 2026

A 3-dimensional 3D-printed Template for High Throughput Zebrafish Embryo Arraying
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3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation.

Lingqian Chang1,2, Stephen Black3, Chandani Chitrakar3

  • 1School of Biological Science and Medical Engineering, Beihang University, Beijing, China. changlingqian1986@buaa.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

Nanoelectroporation (NEP) offers precise, single-cell gene delivery, overcoming limitations of traditional electroporation. This advanced method enhances transfection efficiency and cell viability for genetic material delivery.

Keywords:
DielectrophoresisMagnetic tweezersMicrofluidicsNanochannel arrayNanoelectroporation

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Molecular and Cellular Biology
  • Nanotechnology

Background:

  • Electroporation is a common physical method for gene/drug delivery, allowing efficiency optimization via electric field tuning.
  • Commercial electroporation methods suffer from stochastic transfection and significant cellular damage due to their bulk environment.
  • Existing methods lack precise control, leading to suboptimal outcomes in gene and drug delivery applications.

Purpose of the Study:

  • To introduce nanoelectroporation (NEP) as a novel approach for highly controllable, single-cell gene and drug delivery.
  • To demonstrate the capability of NEP to achieve high transfection efficiency and cellular viability compared to commercial systems.
  • To present a unique device capable of delivering large genetic molecules (>10 kbp) efficiently.

Main Methods:

  • Development of a nanoelectroporation device utilizing a 3D nanochannel array fabricated with cleanroom techniques.
  • Integration of three on-chip high-throughput manipulation technologies: magnetic tweezers (MT), dielectrophoresis (DEP), and thin-film microfluidics.
  • Precise focusing of the electric field onto single cells positioned on nanochannels for controlled electroporation.

Main Results:

  • Nanoelectroporation demonstrated highly controllable, single-cell transfection, achieving significantly higher efficiency than commercial electroporation systems.
  • The NEP system showed improved cellular viability post-transfection, mitigating the damage associated with bulk electroporation.
  • The device successfully delivered large genetic molecules, including DNA and RNA exceeding 10 kbp in size.

Conclusions:

  • Nanoelectroporation represents a significant advancement in gene/drug delivery, offering superior control and efficiency at the single-cell level.
  • The developed NEP device and integrated manipulation technologies provide a robust platform for advanced genetic material delivery.
  • NEP technology holds promise for overcoming the limitations of conventional electroporation, enabling more effective and safer cellular therapies.