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Related Experiment Video

Updated: Apr 8, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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High-Throughput Single-Cell Biochip System for Functional Interrogation of Protein Interactions in Living Cells.

Feng Liu1,2, Chengbao Wu3, Chaojuan Yang1

  • 1School of Engineering Medicine, Beihang University, Beijing, China.

Small (Weinheim an Der Bergstrasse, Germany)
|April 7, 2026
PubMed
Summary

Pixar enables efficient detection of protein-protein interactions (PPIs) in live cells using electric field-based delivery. This platform precisely monitors PPIs in thousands of individual cells, revealing insights into cellular behaviors like migration and proliferation.

Keywords:
DNA probebiochipelectroporationprotein interactionsingle‐cell analysis

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Molecular Interactions

Background:

  • Investigating live-cell protein-protein interactions (PPIs) at single-cell resolution is crucial for understanding cellular heterogeneity.
  • Existing methods for live-cell PPI detection suffer from low delivery efficiency and limited single-cell analysis capabilities.

Purpose of the Study:

  • To develop a novel platform, Pixar, for efficient and precise detection of PPIs in live cells.
  • To enable real-time monitoring of PPIs and their role in cellular behaviors at single-cell resolution.

Main Methods:

  • Utilized focused electric field-based delivery of peptide-tagged protein plasmids and probes for efficient cellular entry (>90% efficiency and viability).
  • Developed a high-throughput single-cell capture array for dynamic PPI profiling.
  • Applied the platform to study the AKT-mTOR protein interaction in regulating cell migration and proliferation.

Main Results:

  • Achieved high delivery efficiency and cell viability (>90%) with the electric field-based method.
  • Successfully profiled the dynamic AKT-mTOR protein interaction in thousands of individual cells.
  • Demonstrated the platform's capability to link PPIs to heterogeneous cellular behaviors.

Conclusions:

  • The Pixar platform offers an efficient and versatile strategy for studying protein function and cellular phenotypes with single-cell precision.
  • Pixar overcomes limitations of conventional PPI detection methods, enabling deeper insights into endogenous regulatory mechanisms.