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Updated: Jan 20, 2026
Preparation and Transformation of Bacteria Using High-Voltage Electroporation
Published on: October 30, 2025
Low-Voltage Flow-Through Electroporation Membrane and Method
Juliette Experton1, Aaron G Wilson1, Charles R Martin2
1Department of Chemistry, University of Florida, Gainesville, FL, USA.
Abstract:
Electroporation uses high electric field gradients to create pores within the membrane of living cells in order to deliver a substance, for example a gene, into the cytoplasm. To achieve such gradients, current electroporation devices deliver voltage pulses in the kV range to the cell medium. We describe here a new device based on gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of magnitude smaller, 4 V. The percentages of electroporated bacteria were found to be more than an order of magnitude higher than obtained with a commercial electroporator.
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