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Related Experiment Video

Updated: Jan 20, 2026

Preparation and Transformation of Bacteria Using High-Voltage Electroporation

Preparation and Transformation of Bacteria Using High-Voltage Electroporation

Published on: October 30, 2025

Low-Voltage Flow-Through Electroporation Membrane and Method.

Juliette Experton¹, Aaron G Wilson¹, Charles R Martin²

¹Department of Chemistry, University of Florida, Gainesville, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 31, 2019

Summary

This summary is machine-generated.

This study introduces a novel gold-microtube membrane device for electroporation, achieving higher bacterial electroporation rates using significantly lower 4V pulses compared to traditional kV devices.

Keywords:

Electric field gradient Electroporation Escherichia coli Gold-microtube membranes

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Published on: November 19, 2009

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Published on: January 7, 2022

Related Experiment Videos

Last Updated: Jan 20, 2026

Preparation and Transformation of Bacteria Using High-Voltage Electroporation

Preparation and Transformation of Bacteria Using High-Voltage Electroporation

Published on: October 30, 2025

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

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Published on: November 19, 2009

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Area of Science:

Biotechnology
Cell Biology
Bioengineering

Background:

Electroporation is a method for delivering substances into cells by creating temporary pores using electric fields.
Conventional electroporation requires high voltage pulses (kV range), posing challenges for cell viability and device complexity.

Purpose of the Study:

To develop a novel electroporation device utilizing gold-microtube membranes.
To demonstrate effective electroporation at significantly reduced voltage levels.
To compare the efficiency of the new device against a commercial electroporator.

Main Methods:

Fabrication of a new electroporation device incorporating gold-microtube membranes.
Application of low voltage pulses (4V) to cell cultures using the novel device.
Quantification of electroporation efficiency by measuring the percentage of electroporated bacteria.
Comparison of results with a standard commercial electroporation system.

Main Results:

The gold-microtube membrane device successfully achieved electroporation at a low voltage of 4V.
Electroporation percentages with the new device were over ten times higher than those achieved with a commercial electroporator.
The novel approach demonstrated superior efficiency in bacterial electroporation.

Conclusions:

Gold-microtube membranes offer a promising platform for developing highly efficient, low-voltage electroporation systems.
This technology has the potential to improve gene delivery and other cellular manipulation techniques.
The developed device represents a significant advancement over existing electroporation methods.