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Related Concept Videos

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection11:40

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Related Experiment Video

Updated: Jan 20, 2026

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
11:40

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Published on: January 21, 2021

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Robotic platform for microinjection into single cells in brain tissue.

Gabriella Shull1,2, Christiane Haffner3, Wieland B Huttner3

  • 1Department of Biomedical Engineering, University of Minnesota, Twin Cities, MN, USA.

EMBO Reports
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

We developed the Autoinjector, a robot for high-throughput single-cell microinjection in brain tissue. This automated system significantly increases yield for studying neural stem cells and their communication.

Keywords:
brain developmentcomputer visionneural stem cellsroboticssingle cell manipulation

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Area of Science:

  • Neuroscience
  • Biotechnology
  • Bioengineering

Background:

  • Microinjection into single brain cells is crucial for studying neural stem cells but is limited by low throughput and required expertise.
  • Current manual methods hinder large-scale analysis of cellular processes within neural tissue.

Purpose of the Study:

  • To develop an automated robotic system, the Autoinjector, for high-throughput, precise microinjection into single cells within brain tissue.
  • To enhance the accessibility and efficiency of single-cell manipulation for neural stem cell research and other applications.

Main Methods:

  • Development of the Autoinjector robot, utilizing microscopic imaging for automated guidance of microinjection needles.
  • Delivery of femtoliter volumes of liquids into single cells, including apical progenitors and newborn neurons.
  • Application in embryonic mouse and human fetal telencephalon tissue, including organotypic slices.

Main Results:

  • The Autoinjector achieved an order of magnitude higher yield compared to manual microinjection, enabling hundreds of cells to be injected per slice.
  • Successfully targeted both apical progenitors and newborn neurons in embryonic and fetal brain tissue.
  • Systematically investigated gap-junctional communication between neural progenitors, identifying apical contact as a feature of coupled cells.

Conclusions:

  • The Autoinjector transforms microinjection into a high-performance, accessible single-cell technique.
  • This platform facilitates high-throughput single-cell analyses in tissue, advancing bioengineering and biophysics.
  • Automated microinjection significantly accelerates research on neural stem cells and intercellular communication.