Related Concept Videos

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier08:40

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous...
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Assay for Blood-brain Barrier Integrity in Drosophila melanogaster09:08

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar...
8.8K
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier09:21

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport...
4.4K
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells09:23

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.
15.4K
The Blood-brain Barrier00:49

The Blood-brain Barrier

Overview
52.5K
Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier09:10

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier

This report provides protocols for assembly, cell culture, and assays on the µSiM platform for the construction of blood-brain barrier...
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