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Related Concept Videos

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its...

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

This video demonstrates the recording of whole-cell currents induced by gamma-aminobutyric acid, or GABA, in a mouse prefrontal cortical brain slice. Controlled delivery of GABA opens chloride channels in a postsynaptic neuron, generating inhibitory postsynaptic currents, or IPSCs. These IPSCs increase with higher GABA concentrations, reflecting GABA's inhibitory activity on synaptic...

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

We use the patch-clamp technique to measure GABA-activated single-channel currents (GABAA channels, GABAA receptors) and the synaptic and tonic currents they generate in neurons. Activation of the channels decreases neuronal excitability in health and disease...

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial...

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

In this article we introduce fast micro-iontophoresis of neurotransmitters as a technique to investigate integration of postsynaptic signals with high spatial and temporal...

Electrophoretic Delivery of GABA into the Epileptic Focus of an Anesthetized Mouse

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Source: Slezia, A. et al. Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice. J. Vis. Exp. (2019)This video demonstrates the electrophoretic delivery of gamma-aminobutyric acid (GABA) to inhibit seizure activity in an anesthetized mouse. It outlines the steps involved in probe implantation, neural activity recording, seizure induction, and controlled GABA delivery via a microfluidic ion pump to assess its therapeutic...

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Related Experiment Video

Updated: Jan 20, 2026

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Published on: October 12, 2017

Cool(ing) brain stem GABA neurons

Ramón A Piñol¹, Marc L Reitman²

¹Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, 20892, USA.

|September 1, 2019

Summary

No abstract available in PubMed .

More Related Videos

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Published on: July 17, 2011

Related Experiment Videos

Last Updated: Jan 20, 2026

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Published on: October 12, 2017

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Published on: July 17, 2011