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Related Experiment Video

Updated: Jan 20, 2026

Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling

Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling

Erythrocyte-Based Pig-a Gene Mutation Assay.

Jeffrey C Bemis¹, Nikki E Hall¹, Stephen D Dertinger²

¹Litron Laboratories, Rochester, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 2, 2019

Summary

This summary is machine-generated.

The Pig-a gene mutation assay detects genotoxicity by identifying red blood cells with a specific cell surface defect. This flow cytometry method is crucial for preclinical safety testing.

Keywords:

Endogenous mutation Erythrocytes Flow cytometry Genotoxicity Glycosylphosphatidylinositol (GPI) anchor High throughput Immunomagnetic separation Pig-a Reticulocytes

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Last Updated: Jan 20, 2026

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Mutation, Gene Flow, and Genetic Drift

Mutation, Gene Flow, and Genetic Drift

Area of Science:

Toxicology
Genetics
Biomedical Science

Background:

Genotoxicity testing is vital in preclinical safety evaluations.
Assessing gene mutations complements chromosomal damage analysis.
The Pig-a gene assay offers a sensitive method for detecting mutations.

Purpose of the Study:

To describe a standardized procedure for the Pig-a gene mutation assay.
To detail the use of MutaFlow® kits for sample processing.
To outline flow cytometric analysis of red blood cells for genotoxicity.

Main Methods:

Utilizes the Pig-a gene mutation assay based on loss of gene function.
Assesses the lack of cell surface protein expression due to GPI anchor defects.
Employs flow cytometry for analyzing red blood cell phenotypes.

Main Results:

The described procedure enables reliable detection of Pig-a gene mutations.
Flow cytometry effectively identifies cells with the specific cell surface phenotype.
MutaFlow® kits facilitate consistent sample processing.

Conclusions:

The Pig-a gene mutation assay is a valuable tool in genotoxicity testing.
Standardized procedures enhance the reliability of preclinical safety assessments.
Flow cytometry provides an efficient method for evaluating red blood cell mutations.