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Related Concept Videos

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies10:13

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies

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Many types of human brain tumors are localized to specific regions within the brain and are difficult to grow in culture. This protocol addresses the role of tumor microenvironment and investigates new drug treatments by analyzing fluorescent primary brain tumor cells growing in an organotypic mouse brain slice.
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The Tumor Microenvironment02:17

The Tumor Microenvironment

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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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Microfluidic Device for Recreating a Tumor Microenvironment in Vitro16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

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We present the procedure for fabrication and operation of a microfluidic device that recreates heterogeneous tumor microenvironments in vitro. The variability in apoptosis within tumor tissue was quantified using fluorescent stains and the effective diffusion coefficient of the chemotherapeutic drug doxorubicin into tumor tissue was...
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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

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Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune...
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A Computational Modeling Approach to Investigate the Influence of Hyperthermia on the Tumor Microenvironment10:23

A Computational Modeling Approach to Investigate the Influence of Hyperthermia on the Tumor Microenvironment

978
The article describes a protocol to simulate the transient temperature profiles and the coupled spatiotemporal variation of the interstitial fluid pressure following the heating delivered by a dipolar radiofrequency hyperthermia system. The protocol can be used to assess the response of biophysical parameters characterizing the tumor microenvironment to interventional hyperthermia techniques.
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Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

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Pancreatic juice is a precious source of biomarkers for human pancreatic cancer. We describe here a method for intraoperative collection procedure. To overcome the challenge of adopting this procedure in murine models, we suggest an alternative sample, tumor interstitial fluid, and describe here two protocols for its...
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Related Experiment Video

Updated: Jan 20, 2026

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
10:13

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies

Published on: November 7, 2015

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Nanotheranostics Targeting the Tumor Microenvironment.

Catarina Roma-Rodrigues1, Inês Pombo1, Luís Raposo1

  • 1UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Costa da Caparica, Portugal.

Frontiers in Bioengineering and Biotechnology
|September 3, 2019
PubMed
Summary
This summary is machine-generated.

Gold nanoparticles are emerging as key nanotheranostics to combat cancer heterogeneity within the tumor micro-environment (TME). These advanced systems offer targeted delivery and diagnostics for improved cancer treatment strategies.

Keywords:
cancer therapydiagnosticgold nanoparticlesnanomedicinenanotheranosticstumor microenvironment

More Related Videos

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
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Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

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Related Experiment Videos

Last Updated: Jan 20, 2026

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
10:13

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies

Published on: November 7, 2015

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Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

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Area of Science:

  • Oncology
  • Nanomedicine
  • Materials Science

Background:

  • Cancer is a leading cause of death globally, characterized by aggressive malignancy and evasion of treatments.
  • Tumor heterogeneity, driven by the tumor micro-environment (TME), complicates conventional therapeutic strategies.
  • Nanomedicine offers innovative solutions to address TME complexity and cancer cell evasion.

Purpose of the Study:

  • To explore the potential of gold nanoparticles in developing nanotheranostics for cancer treatment.
  • To highlight the role of gold nanoparticles in addressing tumor micro-environment heterogeneity.
  • To review recent advancements in gold nanoparticle-based nanotheranostics for cancer therapy.

Main Methods:

  • Review of recent developments in nanomedicine and nanotheranostics.
  • Focus on the application of gold nanoparticles in cancer therapy.
  • Analysis of gold nanoparticles' properties for multifunctional nanomedicines.

Main Results:

  • Gold nanoparticles exhibit promising physicochemical properties for nanomedicine.
  • Nanotheranostics based on gold nanoparticles enable targeted delivery and diagnostics.
  • These systems are suitable for combinatory therapy and addressing cancer heterogeneity.

Conclusions:

  • Gold nanoparticles are pivotal in developing advanced nanotheranostics.
  • Nanotheranostics offer a selective approach to target cancer cells and the TME.
  • This strategy holds significant potential for overcoming challenges in cancer treatment and heterogeneity.