Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single Crystal and Powder X-ray Diffraction08:14

Single Crystal and Powder X-ray Diffraction

108.4K
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University 
X-ray crystallography is a technique that uses X-rays to study the structure of molecules. X-ray diffraction (XRD) experiments are routinely carried out with either single-crystal or powdered samples.
Single-crystal XRD:
Single-crystal XRD allows for absolute structure determination. With single-crystal XRD data, the exact atomic positions can be observed, and thus bond lengths and angles can be determined. This...
108.4K
Growing Crystals for X-ray Diffraction Analysis08:00

Growing Crystals for X-ray Diffraction Analysis

33.5K
Source: Laboratory of Dr. Jimmy Franco - Merrimack College
X-ray crystallography is a method commonly used to determine the spatial arrangement of atoms in a crystalline solid, which allows for the determination of the three-dimensional shape of a molecule or complex. Determining the three-dimensional structure of a compound is of particular importance, since a compound's structure and function are intimately related. Information about a compound's structure is often used to explain its...
33.5K
X-ray Diffraction09:31

X-ray Diffraction

92.9K
Source: Faisal Alamgir, School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA
X-ray diffraction (XRD) is a technique used in materials science for determining the atomic and molecular structure of a material. This is done by irradiating a sample of the material with incident X-rays and then measuring the intensities and scattering angles of the X-rays that are scattered by the material. The intensity of the scattered X-rays are plotted as a function of the...
92.9K
Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

7.0K
This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the...
7.0K
High Pressure Single Crystal Diffraction at PX^211:32

High Pressure Single Crystal Diffraction at PX^2

22.1K
In this report, we describe detailed procedures for carrying out single crystal X-ray diffraction experiments with a diamond anvil cell at the GSECARS 13-BM-C beamline at the Advanced Photon Source. ATREX and RSV programs are used to analyze the...
22.1K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

15.2K
This protocol describes in detail how to fabricate and operate microfluidic devices for X-ray diffraction data collection at room temperature. Additionally, it describes how to monitor protein crystallization by dynamic light scattering and how to process and analyze obtained diffraction...
15.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The limit of application of the Scherrer equation.

Acta crystallographica. Section A, Foundations and advances·2017
Same author

Structural analysis of zeolite NaA synthesized by a cost-effective hydrothermal method using kaolin and its use as water softener.

Journal of colloid and interface science·2011
Same author

In situ X-ray diffraction study on the growth kinetics of NiO nanoparticles.

Journal of synchrotron radiation·2010
Same author

In situ system for X-ray absorption spectroscopy experiments to investigate nanoparticle crystallization.

Journal of synchrotron radiation·2006
Same author

Raman spectra of deuteriated taurine single crystals.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2005
Same journal

Case study of using the single-atom R1 method to solve a small protein structure.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

Beyond complementarity: a reverse-engineering framework for de novo crystal structure determination from EXAFS.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

Crystallography in Open Science and its open educational resources.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

From atoms to a data bank: optimizing transferability of electron-density symmetry.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

Twenty-Sixth General Assembly and International Congress of Crystallography, Melbourne, Australia, 22-29 August 2023.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

MIDAS: a quantitative framework for high-energy diffraction microscopy. Part II: accuracy, robustness and best practices.

Acta crystallographica. Section A, Foundations and advances·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Single Crystal and Powder X-ray Diffraction
08:14

Single Crystal and Powder X-ray Diffraction

Published on: April 30, 2023

108.4K

X-ray diffraction in superabsorbing crystals: absorption intrinsic width.

A N C Lima1, M A R Miranda2, J M Sasaki2

  • 1Departamento de Engenharia Metalúrgica e de Materiais, Universidade Federal do Ceará, CEP 60455-760 Fortaleza, Ceará, Brazil.

Acta Crystallographica. Section A, Foundations and Advances
|September 3, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a simple mathematical formulation for X-ray diffraction in superabsorbing crystals, crucial for advanced materials characterization techniques like soft resonant X-ray diffraction.

Keywords:
absorption intrinsic widthpeak profilesoft X-ray diffractionsuperabsorbing crystals

More Related Videos

Growing Crystals for X-ray Diffraction Analysis
08:00

Growing Crystals for X-ray Diffraction Analysis

Published on: April 30, 2023

33.5K
X-Ray Diffraction for Determining Atomic and Molecular Structure
09:31

X-Ray Diffraction for Determining Atomic and Molecular Structure

Published on: April 30, 2023

92.9K

Related Experiment Videos

Last Updated: Jan 20, 2026

Single Crystal and Powder X-ray Diffraction
08:14

Single Crystal and Powder X-ray Diffraction

Published on: April 30, 2023

108.4K
Growing Crystals for X-ray Diffraction Analysis
08:00

Growing Crystals for X-ray Diffraction Analysis

Published on: April 30, 2023

33.5K
X-Ray Diffraction for Determining Atomic and Molecular Structure
09:31

X-Ray Diffraction for Determining Atomic and Molecular Structure

Published on: April 30, 2023

92.9K

Area of Science:

  • Materials Science
  • Crystallography
  • Condensed Matter Physics

Background:

  • X-ray diffraction (XRD) is a key materials characterization technique.
  • Advancements in synchrotrons necessitate simpler XRD formulations for new techniques.
  • Soft resonant X-ray diffraction experiences significant X-ray attenuation.

Purpose of the Study:

  • To derive a simplified mathematical formulation for X-ray diffraction profiles in superabsorbing crystals.
  • To analyze the diffraction profiles of parallel plate crystals with high linear absorption.
  • To provide new perspectives for soft resonant X-ray diffraction analysis.

Main Methods:

  • Derivation of an expression for X-ray diffraction profiles in superabsorbing crystals.
  • Computation of diffraction profiles as a function of crystal size.
  • Comparison with kinematical theory and Laue dynamical theory.

Main Results:

  • For thin or low-absorption crystals, profiles match kinematical theory.
  • For thick crystals, an absorption intrinsic profile (Lorentzian) is observed.
  • The absorption intrinsic width is proportional to the linear absorption coefficient.

Conclusions:

  • The derived formulation simplifies the analysis of X-ray diffraction in superabsorbing crystals.
  • This work offers new insights into soft resonant X-ray diffraction.
  • The formulation bridges kinematical and dynamical theories for highly absorbing materials.