Hyb-Seq for Flowering Plant Systematics

Flowers are the reproductive, seed-producing structures of angiosperms. Typically, flowers consist of sepals, petals, stamens, and carpels. Sepals and petals are the vegetative flower organs. Stamens and carpels are the reproductive organs.  

In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive...

Presented here is a protocol to determine the number of thrips and minute pirate bug predators in crops over multiple dates in field experiments. Also illustrated is how to determine the efficacy of management tactics against thrips and evaluate the benefits of predation by minute pirate...

Here, we present a protocol to force flowering in mandarin trees under phytotron conditions. Water stress, high illuminance and a simulated spring photoperiod allowed viable flowers to be obtained in a short period of time. This methodology allows researchers to have several flowering periods in 1 year.

Live confocal imaging provides biologists with a powerful tool to study development. Here, we present a detailed protocol for the live confocal imaging of developing Arabidopsis...

Viewing Prepared Slides
ExpandTo start the exercise, first split up into groups and find a station with a compound microscope, dissecting microscope and one prepared slide.
HYPOTHESES: The alternative hypothesis for this experiment might be that plants have specific adaptations that allow them to survive and reproduce in their own individual terrestrial environments. The null hypothesis for this experiment is that all plants have the same adaptations to live in terrestrial habitats.
Find the...