Topological magnon amplification

Daniel Malz1, Johannes Knolle2, Andreas Nunnenkamp3

  • 1Max-Planck-Institut für Quantenoptik, Hans-Kopfermann-Strasse 1, D-85748, Garching, Germany. daniel.malz@mpq.mpg.de.

Nature Communications
|September 4, 2019
PubMed

Related Concept Videos

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)08:14

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

Here, we present a protocol to determine the orientation and topology of integral membrane proteins in living cells. This simple protocol relies on selective protease sensitivity of chimeras between the protein of interest and GFP.
18.3K
Assessing Immunological Synapse Topology through Live-Cell Imaging05:03

Assessing Immunological Synapse Topology through Live-Cell Imaging

This video demonstrates live-cell imaging of immunological synapse topology, investigating the interactions between T lymphocytes and epithelial cells carrying fluorescent antigens. Fluorescence microscopy reveals red cytoplasm displacement and yellow membrane rings in endothelial cells, indicating the formation of immunological...
527
Rapid Amplification of cDNA Ends09:44

Rapid Amplification of cDNA Ends

Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3
1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research
2Department of Cell and Tissue Biology, 
3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA    
Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior...
16.1K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.2K
Amplification of a Suicide Plasmid in a Bacterial Host02:41

Amplification of a Suicide Plasmid in a Bacterial Host

Source: Tomás, J. M., et al. Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility. J. Vis. Exp. (2022).This video demonstrates the initiator-dependent amplification of a suicide plasmid in E. coli. The plasmid, which carries an antibiotic resistance gene, replicates only in the presence of an initiator protein encoded by the host. After electroporation and incubation in recovery medium, the mixture is plated on antibiotic-containing agar.
275
Loop-Mediated Isothermal Amplification for Bacterial Gene Detection02:48

Loop-Mediated Isothermal Amplification for Bacterial Gene Detection

Source: Domesle, K. J., et al. Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation. J. Vis. Exp. (2020).This video demonstrates the loop-mediated isothermal amplification (LAMP) technique for detecting a target bacterial gene by amplifying DNA at a constant temperature. The reaction mixture is prepared by adding an isothermal master mix, specific primers for the target gene, and samples containing DNA templates. The...
201