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ALS - Motor Neuron Disease: Mechanism and Development of New Therapies15:48

ALS - Motor Neuron Disease: Mechanism and Development of New Therapies

Jeffrey D. Rothstein speaks about the pathology and mechanisms underlying amyotrophic lateral sclerosis or ALS, advances in ALS research, and current strategies towards the development of...
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A Preclinical Model of Sepsis-Induced Myopathy with Disuse in Mice04:01

A Preclinical Model of Sepsis-Induced Myopathy with Disuse in Mice

This murine model combines a septic insult with hindlimb muscle disuse to recapitulate the bedridden feature of the typical septic patient. The model represents a significant departure from previous models to study muscle dysfunction in sepsis and is a reproducible approach to addressing therapeutic strategies to treat this...
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Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica13:37

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

In animals with large identified neurons (e.g. mollusks), analysis of motor pools is done using intracellular techniques1,2,3,4. Recently, we developed a technique to extracellularly stimulate and record individual neurons in Aplysia californica5. We now describe a protocol for using this technique to uniquely identify and characterize motor neurons within a motor...
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Intramuscular Injections Along the Motor End Plates to Shuttle Tracers Directly into Rat Motor Neurons03:31

Intramuscular Injections Along the Motor End Plates to Shuttle Tracers Directly into Rat Motor Neurons

Source: Mohan, R., et al. Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons. J. Vis. Exp. (2015)This video demonstrates the targeted delivery of a fluorescent neuronal tracer into motor neurons via intramuscular injection at the motor end plates (MEPs). It outlines the steps involved in preparing the rat, injecting the tracer, and assessing labeled motor neurons for neurotherapeutic...
413
Preparation of Aplysia Sensory-motor Neuronal Cell Cultures17:27

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

Primary cultures of Aplysia sensory-motor neurons provide a model preparation for studying synapse formation and synaptic plasticity in vitro. This video demonstrates the identification and microdissection of sensory and motor neurons from Aplysia ganglia as well as the methods for establishing and maintaining sensory-motor neurons in...
16.4K
Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons10:57

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons

The efficacy of intramuscular uptake and retrograde transport of molecules to corresponding motor neurons depends on the location of the injection sites with respect to the motor end plates (MEPs). Here, we describe how to locate MEPs on skeletal muscles to optimise retrograde transport of tracers into motor...
13.1K