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Updated: Jan 20, 2026

Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
Duplex-forming Oligonucleotide of Triazole-linked RNA
Tomoko Fujino1,2, Takeru Suzuki3, Tsugumi Ooi1
1Department of Chemistry, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Abstract:
An oligonucleotide of triazole-linked RNA (TL RNA) was synthesized by performing consecutive copper-catalyzed azide-alkyne cycloaddition reactions for elongation. The reaction conditions that had been optimized for the synthesis of 3-mer TL RNA were found to be inappropriate for longer oligonucleotides, and the conditions were reoptimized for the solid-phase synthesis of an 11-mer TL RNA oligonucleotide. Duplex formation of the 11-mer TL RNA oligonucleotide was examined with the complementary oligonucleotide of natural RNA to reveal the effects of the 2'-OH groups on the duplex stability.
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