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Related Experiment Video

Updated: Jan 20, 2026

&beta;-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions
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New Trimodal Phenotypic Reporter of Extended-Spectrum β-Lactamase Activity.

Hieu T Nguyen1, Shweta Ganapati2, David Watts2

  • 1Fischell Department of Bioengineering , University of Maryland , 8278 Paint Branch Drive , College Park , Maryland 20742 , United States.

ACS Infectious Diseases
|September 4, 2019
PubMed
Summary

A new glucose-releasing substrate detects extended-spectrum β-lactamases (ESBLs) in bacterial infections. This rapid test aids antibiotic stewardship by distinguishing dangerous ESBLs from older enzymes, preserving last-resort antibiotics.

Keywords:
ESBLbiosensorcephalosporinmultidrug resistance

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Area of Science:

  • Microbiology
  • Biochemistry
  • Clinical Diagnostics

Background:

  • Bacterial resistance to β-lactam antibiotics is a growing global health threat.
  • Extended-spectrum β-lactamases (ESBLs) confer resistance to advanced cephalosporins and other critical antibiotics.
  • Accurate and rapid detection of ESBLs is crucial for effective antibiotic stewardship.

Purpose of the Study:

  • To develop a novel phenotypic detection substrate for rapid and specific identification of ESBLs.
  • To enable ESBL quantification using multiple common clinical laboratory detection modalities.
  • To engineer a substrate resistant to older β-lactamases for enhanced specificity.

Main Methods:

  • Design and synthesis of a glucose-releasing β-lactamase substrate.
  • Quantification of ESBL activity using optical absorbance, fluorescence, and electrochemical detection.
  • Testing substrate specificity against various β-lactamase enzymes.

Main Results:

  • The novel substrate successfully detects ESBL hydrolysis by releasing glucose.
  • The substrate is quantifiable via absorbance, fluorescence, and electrochemistry.
  • Engineered substrate demonstrates resistance to older β-lactamases, enabling specific ESBL detection.

Conclusions:

  • The developed substrate offers a versatile and specific tool for rapid ESBL detection.
  • This advancement can significantly improve antibiotic stewardship and patient outcomes.
  • The multi-modal detection capability allows for broad clinical laboratory adoption.