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Enhanced Intraliposomal Metallic Nanoparticle Payload Capacity Using Microfluidic-Assisted Self-Assembly.

Zahraa S Al-Ahmady1,2,3, Roberto Donno3,4, Arianna Gennari3,4

  • 1Nanomedicine Lab, Division of Pharmacy and Optometry, Faculty of Biology, Medicine and Health , University of Manchester , Av Hill Building , Manchester M13 9PT , U.K.

Langmuir : the ACS Journal of Surfaces and Colloids
|September 4, 2019
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Summary

Microfluidics enables efficient fabrication of liposome-gold nanoparticle (AuNP) hybrids with high loading capacity. This advanced method improves nanoparticle incorporation for enhanced imaging and drug delivery applications.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Liposome-nanoparticle (NP) hybrids are promising for imaging and drug delivery.
  • Efficiently incorporating metallic NPs into liposomes remains a significant challenge with conventional methods.

Purpose of the Study:

  • To develop a microfluidic-assisted self-assembly process for fabricating liposome-gold nanoparticle (AuNP) hybrids.
  • To compare the efficiency and characteristics of AuNP-loaded liposomes produced via microfluidics versus the reverse-phase evaporation method.

Main Methods:

  • Fabrication of liposome-hydrophobic gold NP hybrids using microfluidics.
  • Comparison with hybrids prepared by the reverse-phase evaporation method.
  • Characterization using ultraviolet spectroscopy, ICP-MS, FFF, TEM, SAXS, fluorescent anisotropy, AFM, and cryo-EM.

Main Results:

  • Microfluidics yielded AuNP-liposome hybrids with homogeneous size distribution and lower polydispersity.
  • A threefold increase in loading efficiency was observed with the microfluidic method.
  • Higher AuNP loading resulted in a thicker, more rigid liposome bilayer, with NPs embedded within or forming interdigitated domains.

Conclusions:

  • Microfluidics offers an automated, efficient method for producing monodisperse liposome-NP hybrids with high loading capacity.
  • This technique significantly enhances the payload of metallic NPs within liposomes.
  • The improved liposome-NP hybrids show enhanced potential for advanced imaging and drug delivery applications.