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A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients09:28

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of...
12.6K
Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Restricting the timing of food intake has emerged as a promising intervention to attenuate diet-induced metabolic diseases. This manuscript details the construction and use of an efficient system built in-house for measuring and manipulating rhythmic food intake in...
2.8K
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Here we are presenting a chromatin immunoprecipitation (ChIP) procedure for genome-wide location analysis of protein isoforms that differ in a histone-binding domain. We are applying it to ChIP-Seq analysis to identify the targets of the KDM5A/JARID1A/RBP2 histone...
15.0K
Synthesis of an Intein-mediated Artificial Protein Hydrogel15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

We present the synthesis of a split-intein-mediated protein hydrogel. The building blocks of this hydrogel are two protein copolymers each containing a subunit of a trimeric protein that serves as a crosslinker and one half of a split intein. Mixing of the two protein copolymers triggers an intein trans-splicing reaction, yielding a polypeptide unit that self-assembles into a hydrogel. This hydrogel is highly pH- and temperature-stable, compatible with organic solvents, and easily incorporates...
12.7K
Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals08:54

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals

A two dimensional model material of discotic zirconium phosphate was developed. The inorganic crystal with lamellar structure was synthesized by hydrothermal, reflux, and microwave-assisted methods. On exfoliation with organic molecules, layered crystals can be converted to monolayers, and nematic liquid crystal phase was formed at sufficient concentration of monolayers.
8.9K
Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions12:25

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

This protocol describes a microfluidic co-culture model for simultaneous and localized culture of epithelial cells and bacteria. This model can be used for investigating the role of different soluble molecular signals on pathogenesis as well as screen the effectiveness of putative probiotic bacterial...
14.5K