Detailed analysis at a single-cell level of cells undergoing pancreatic differentiation

Pancreatic cells derived from pluripotent stem cells using the stem cell-derived-β (SC-β) differentiation protocol are composed of four main cell types, namely β-like cells (SC-β-cells), α-like cells, enterochromaffin-like cells and non-endocrine cells. Single-cell dissociation and reaggregation depleted non-endocrine cells and improved β-cell function. Veres et al. succeeded in efficiently purifying SC-β by magnetic cell sorting using CD49a antibody and reaggregation, so that the final SC-β-cell ratio increased to 80%.