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Related Experiment Video

Updated: Jan 20, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

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A High-Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity.

Susana Rosa1, Patrícia Pitrez2, Hugo Fernandes2

  • 1CNC-Center for Neurosciences and Cell Biology, University of Coimbra, Rua Larga, Coimbra, Portugal.

Current Protocols in Stem Cell Biology
|September 4, 2019
PubMed
Summary

Researchers developed a novel screening platform using human embryonic-like endothelial cells to identify compounds affecting embryonic vascular development, offering an alternative to animal testing.

Keywords:
drug discoveryhigh-throughput screeningstem cell biotechnologytissue engineeringtoxicology

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Related Experiment Videos

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Area of Science:

  • Developmental Biology
  • Toxicology
  • Stem Cell Biology

Background:

  • Assessing the impact of chemical compounds on human embryonic vascular development is crucial for safety.
  • Existing animal-based protocols can be time-consuming and ethically complex.

Purpose of the Study:

  • To establish a high-throughput screening platform for identifying compounds that disrupt human embryonic vascular development.
  • To provide an alternative to animal testing for evaluating chemical safety during embryonic development.

Main Methods:

  • Generation and maturation of human embryonic-like endothelial cells (ECs) from human pluripotent stem cells (hPSCs) under arterial flow conditions.
  • High-throughput screening of small molecules for inhibition of embryonic-like EC survival.
  • Confirmation of hits using ECs under flow shear stress and validation in mouse embryonic ECs.

Main Results:

  • The platform successfully identified compounds affecting embryonic-like EC survival.
  • Embryonic-like ECs expressed key embryonic genes (e.g., DLL1, EPHB2, FLT1).
  • The screening procedure, excluding validation, can be completed within one month.

Conclusions:

  • The developed platform offers a rapid and effective method for assessing chemical effects on embryonic vascular development.
  • This in vitro system serves as a valuable complement or alternative to traditional animal testing protocols.