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Updated: Jan 20, 2026

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates
Published on: November 5, 2008
Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish
Takeshi Ikeda1,2, Kohei Ichikawa2, Hajime Shigeto2,3
1Unit of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
Abstract:
Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.
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