Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

584
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
584
Comet Assay for DNA Damage Detection in Cells06:19

Comet Assay for DNA Damage Detection in Cells

2.8K
The video demonstrates the comet assay for detecting DNA damage following exposure to harmful agents. Under alkaline conditions, the DNA unwinds and denatures, and upon electrophoresis, the undamaged and damaged DNA migrate differently in the agarose gel, forming a comet-shaped structure. The slowly migrating undamaged DNA forms the circular comet head, while the rapidly migrating damaged DNA fragments form the elongated comet...
2.8K
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts05:58

Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts

4.3K
This protocol uses an immunofluorescence assay to detect PM2.5-induced DNA damage in the dissected hearts of zebrafish...
4.3K
Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method09:54

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

16.8K
This video describes dissection, tissue processing, sectioning, and fluorescence-based TUNEL labeling of mouse skeletal muscle. It also describes a method for semi-automated analysis of TUNEL labeling.
16.8K
Immunofluorescence Assay to Detect PM2.5-Mediated DNA Damage in Fish Embryos04:57

Immunofluorescence Assay to Detect PM2.5-Mediated DNA Damage in Fish Embryos

557
This video demonstrates a highly sensitive immunofluorescence-based assay for detecting PM2.5-induced oxidative damage in the hearts of zebrafish embryos by measuring the levels of 8-OHdG and γH2AX, which indicate DNA damage and double-strand DNA breaks,...
557
Comet Assay to Quantify DNA Damage in FLT3 Mutant-expressing 32D Cells after Exposure to Type I and Type II FLT3 Inhibitors04:36

Comet Assay to Quantify DNA Damage in FLT3 Mutant-expressing 32D Cells after Exposure to Type I and Type II FLT3 Inhibitors

853
This protocol describes an effective method for quantifying the difference in DNA damage induced by type I and type II inhibitors in FLT3 mutant cells through the application of the comet...
853

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic Liquid Biopsy Enables Universal Mutation-Agnostic Molecular Surveillance for High-Risk Neuroblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Sequencing-free On-chip detection of lung cancer by fluorescent enzymatic profiling of cfDNA methylation.

NPJ precision oncology·2026
Same author

Cigarette smoke induces FASN-dependent fatty acid metabolic rewiring to drive bladder cancer progression.

Cancer letters·2026
Same author

Parkinson's disease linked LRRK2 G2019S drives oxidative nuclear DNA damage and PARP1 hyperactive signaling.

bioRxiv : the preprint server for biology·2026
Same author

Oxalate induces proliferation and mitochondrial metabolism in select clear cell renal cell carcinoma cell lines.

BMC cancer·2026
Same author

Compact Spectral Encoding Microscopy by Terrace Grating Optics.

ACS photonics·2026

Related Experiment Video

Updated: Jan 20, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

584

Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.

Dmitry Torchinsky1, Yael Michaeli, Natalie R Gassman

  • 1Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv 6997801, Israel. uv@post.tau.ac.il.

Chemical Communications (Cambridge, England)
|September 5, 2019
PubMed
Summary

This study introduces a new assay to detect oxidative DNA damage and photoproducts simultaneously in skin cells. The method uses DNA repair enzymes and fluorescent nucleotides to track DNA lesion repair after artificial sunlight exposure.

More Related Videos

Comet Assay for DNA Damage Detection in Cells
06:19

Comet Assay for DNA Damage Detection in Cells

2.8K
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
05:58

Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts

Published on: February 15, 2021

4.3K

Related Experiment Videos

Last Updated: Jan 20, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

584
Comet Assay for DNA Damage Detection in Cells
06:19

Comet Assay for DNA Damage Detection in Cells

2.8K
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
05:58

Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts

Published on: February 15, 2021

4.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA damage from oxidative stress and UV radiation poses significant health risks.
  • Existing methods often struggle to simultaneously quantify diverse DNA lesions.
  • Understanding DNA repair mechanisms is crucial for preventing diseases like cancer.

Purpose of the Study:

  • To develop and validate a novel assay for concurrent detection of oxidative DNA damage and photoproducts.
  • To enable simultaneous quantification of multiple DNA lesions on individual DNA molecules.
  • To investigate the kinetics of DNA repair for both damage types in cellular models.

Main Methods:

  • Utilized DNA repair enzymes specific for oxidative lesions and photoproducts.
  • Incorporated spectrally distinct fluorescent nucleotides to label repaired sites.
  • Employed single-molecule detection techniques for precise quantification.
  • Exposed human skin cells to artificial sunlight to induce DNA damage.

Main Results:

  • Successfully demonstrated concurrent detection of oxidative DNA damage and photoproducts.
  • Quantified the levels of individual DNA lesions on a single-molecule basis.
  • Monitored the repair dynamics of both damage types in real-time within exposed cells.

Conclusions:

  • The developed assay provides a powerful tool for studying complex DNA damage and repair.
  • This method allows for a more comprehensive understanding of cellular responses to genotoxic agents.
  • Findings contribute to the fields of DNA repair, photobiology, and toxicology.