Pseudomonas tructae sp. nov., novel species isolated from rainbow trout kidney

This study describes the biochemical and phylogenetic characteristics of a Gram-negative strain, SNU WT1^T, isolated from rainbow trout kidney. The 16S rRNA gene sequencing indicated that strain SNU WT1^T was highly similar to Pseudomonas wadenswilerensis CCOS 864^T and closely related to other Pseudomonas putida-related strains. Multilocus sequence analysis of concatenated partial gyrB, rpoB and rpoD sequences revealed that strain SNU WT1^T was distinct from P. putida-related strains and formed a separate clade. The average nucleotide identity and Genome-to-Genome Distance Calculator values were 90.19 and 41.7 %with its closest relative P. wadenswilerensis CCOS 864^T; however, it was phenotypically distinct from CCOS 864^T with respect to arginine dihydrolase, glucose fermentation, aesculin hydrolysis and N-acetyl-glucosamine assimilation. The major polar lipid of the strain was phosphatidylethanolamine and the major quinone was Q-9. The genome of strain SNU WT1^T had 5 685 196 bp with a G+C content of 61.83 mol%. We describe a novel species of genus Pseudomonas, for which the name Pseudomonastructae has been proposed, with SNU WT1^T (=KCTC 72265=JCM 33436) as the type strain.