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Related Experiment Video

Updated: Jan 20, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
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Turning DNA Binding Motifs into a Material for Flow Cells.

Tobias Feldner1, Manpreet Wolfrum1, Clemens Richert1

  • 1Institut für Organische Chemie, Universität Stuttgart, Pfaffenwaldring 55, 70569, Stuttgart, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|September 5, 2019
PubMed
Summary

Researchers developed a novel DNA-based solid material for reversible cofactor binding and controlled release. This innovation enables efficient recycling of expensive biochemical reagents in flow systems, demonstrated using ATP-induced bioluminescence.

Keywords:
DNAcofactorsflow cellnanostructurestriplexes

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Area of Science:

  • Biomolecular Engineering
  • Materials Science
  • Biochemistry

Background:

  • Designing nanoscale DNA assemblies for specific functions is feasible.
  • Creating solid-state materials from DNA for reversible biomolecule binding, particularly cofactors, presents a significant challenge.
  • Cofactors are crucial and expensive reagents in biochemical transformations, necessitating methods for controlled release and recycling.

Purpose of the Study:

  • To develop a macroporous solid material from DNA triplex motifs for reversible binding of adenine-containing cofactors.
  • To evaluate the suitability of the developed material for use in flow cell applications.
  • To demonstrate controlled loading, discharge, and cofactor-mediated biological activity using the material.

Main Methods:

  • Utilized DNA triplex motifs with known affinity for adenine-containing cofactors (NAD, FAD, ATP).
  • Investigated methods to convert soluble DNA motifs into a macroporous solid, comparing hydrogels from linear/branched motifs with long triplexes treated with protamine.
  • Employed exchangeable cells in a flow system with thermal control for cofactor loading and discharge.
  • Demonstrated ATP release and induction of bioluminescence in a flow cell.

Main Results:

  • Long DNA triplexes treated with protamine formed suitable macroporous solids for flow cells, unlike other DNA assemblies.
  • Thermally controlled loading and discharge of cofactors were successfully demonstrated in an exchangeable flow system.
  • ATP release from the functional DNA material induced bioluminescence, confirming cofactor activity.

Conclusions:

  • Functional DNA structures can be successfully converted into solid materials for use in macroscopic devices.
  • The developed DNA-based material offers a promising platform for reversible cofactor binding and controlled release.
  • This approach has potential applications in recycling expensive biochemical reagents and developing advanced biosensors or biocatalytic systems.