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This protocol separates insoluble cell wall and membrane proteins into simple fractions (1-5 proteins) using preparative isoelectric focusing (IEF) based on isoelectric point, followed by separation by molecular weight. The resulting fractions can be used directly for immunological and proteomic analysis without further...
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A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules10:26

A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules

The objective of this study is to develop a novel 3D digital model of pulmonary nodules that serves as a communication bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic...
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We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine...
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A Retrospective Study on Endoscopic Surgery for the Treatment of Paravertebral Abscess in Spinal Tuberculosis Patients03:47

A Retrospective Study on Endoscopic Surgery for the Treatment of Paravertebral Abscess in Spinal Tuberculosis Patients

This study evaluated endoscopic debridement for treating paravertebral abscesses in patients with spinal tuberculosis. Compared to conventional surgery, it significantly improved surgery time, blood loss, hospital stay, pain relief, and neurological outcomes, with fewer complications and no recurrences. These findings highlight its efficacy and safety. Further long-term studies are...
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The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis23:06

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture...
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