Related Experiment Video

Updated: Jan 20, 2026

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
09:25

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

Published on: October 1, 2007

11.8K

Membrane Lipid Remodeling Takes Center Stage in Growth Factor Receptor-Driven Cancer Development

Johannes V Swinnen1, Jonas Dehairs1, Ali Talebi1

  • 1Laboratory of Lipid Metabolism and Cancer, Department of Oncology, LKI - Leuven Cancer Institute, KU Leuven, 3000 Leuven, Belgium.

Cell Metabolism
|September 5, 2019
PubMed

More Related Videos

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy09:16

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

Published on: September 11, 2015

8.7K
Crystallization of Membrane Proteins in Lipidic Mesophases11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

31.8K

Related Concept Videos

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos09:25

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

A variety of growth factors and proteins interact to induce cells to take on different cell fates during development. Here we demonstrate the use of an in ovo preparation to address possible interactions between different proteins in development by placing beads on E2.5 chick...
11.8K
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy09:16

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.
8.7K
Crystallization of Membrane Proteins in Lipidic Mesophases11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting...
31.8K
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic...
30.6K
Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)

This protocol utilizes agarose swelling as a powerful and generalizable technique for incorporating integral membrane proteins (IMPs) into giant unilamellar lipid vesicles (GUVs), as described here for the reconstitution of the human 1A serotonin receptor protein (5-HT1AR), one of the classes of pharmacologically important G protein-coupled...
4.0K
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

We present a technique for contactless micromanipulation of vesicles, using localized calcium ion gradients. Microinjection of a calcium ion solution, in the vicinity of a giant lipid vesicle, is utilized to remodel the lipid membrane, resulting in the production of membrane tubular...
8.3K