Inflammatory Mediators in Obesity

This video demonstrates the quantification of inflammatory mediators during infection by incubating human lung-tissue-derived immune cells with Haemophilus influenzae. Flow cytometry is used to measure cytokine fluorescence in labeled lymphocytes, correlating with the production of pro-inflammatory cytokines in response to bacterial...

Take cultures of human monocyte-derived microglia-like cells.Introduce nanoparticles containing an anti-inflammatory drug into the experimental culture and the free drug into the control.The nanoparticles feature a hydrophilic shell and a hydrophobic core encapsulating the lipophilic drug.The cells endocytose the nanoparticles, which release the drug intracellularly, enhancing its delivery.The free drug enters by passive diffusion, a process constrained by the concentration gradient, which...

A technique is presented for the in-vivo collection of interstitial fluid samples from pertinent tissue sites (here, experimentally inflamed skin) for the measurement of biochemicals mediating pain and...

The Body Mass Index (BMI) is a numerical value derived from a person's weight and height, used to categorize individuals into weight ranges. It is calculated using the formula: weight in kilograms divided by height in meters squared. Obesity is a health condition characterized by excessive accumulation of adipose tissue that poses health risks, often diagnosed with a BMI ≥ 30. This excess fat storage occurs when surplus dietary calories are converted into triglycerides and stored in...

This protocol allows a researcher to isolate and characterize tissue-resident macrophages in various hallmark inflamed tissues extracted from diet-induced models of metabolic...

Obesity care is best delivered after in-depth initial screening and evaluation, using an individualized approach comprising multidisciplinary care, evidence-based lifestyle behavioral change strategies, and frequent follow-up. We illustrate a successful 6 month course of weight loss treatment, resulting in reversal of obesity-related chronic conditions and improvement in diet, sleep, mood, and physical fitness.