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Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats04:16

Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats

This article describes methods for subretinal injection in rats, utilizing intraoperative visualization to control both the injection site and the area of retinal...
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Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate05:58

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

Creating experimental retinal detachments with a reproducible and sustained height of detachment, and without subretinal hemorrhage, is important for studying the pathophysiology of photoreceptor cell loss in retinal disease and evaluating potential therapeutic interventions. Here, we report such a method in...
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A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion05:20

A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion

Here, we describe a mouse model of retinal ischemia by transient bilateral common carotid artery occlusion using simple sutures and a clamp. This model can be useful for understanding the pathological mechanisms of retinal ischemia caused by cardiovascular...
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Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases11:20

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

The non-human primate (NHP) is an ideal model for studying human retinal cellular therapeutics due to the anatomical and genetic similarities. This manuscript describes a method for submacular transplantation of retinal pigment epithelial cells in the NHP eye and strategies to prevent intraoperative complications associated with macular...
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Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry05:25

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and...
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Preparation of Retinal Samples for Volume Electron Microscopy03:48

Preparation of Retinal Samples for Volume Electron Microscopy

This protocol describes the detailed steps for preparing retinal samples for volume electron microscopy, focusing on the structural features of retinal photoreceptor...
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