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Related Concept Videos

Purification of Progenitors from Skeletal Muscle12:55

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We present techniques for isolating, culturing, characterizing, and differentiating human primary muscle progenitor cells (hMPCs) obtained from skeletal muscle biopsy tissue. hMPCs obtained and characterized through these methods can be used to subsequently address research questions related to human myogenesis and skeletal muscle...
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Related Experiment Video

Updated: Jan 20, 2026

Purification of Progenitors from Skeletal Muscle
12:55

Purification of Progenitors from Skeletal Muscle

Published on: March 16, 2011

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Skeletal Muscle Progenitor Cell Heterogeneity.

Dong Seong Cho1, Jason D Doles2

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.

Advances in Experimental Medicine and Biology
|September 6, 2019
PubMed
Summary

Skeletal muscle regeneration relies on diverse stem cells, particularly satellite cells (SCs). Understanding SC heterogeneity is key to unlocking insights into muscle repair, aging, and disease.

Keywords:
Asymmetric divisionCell cycleCellular heterogeneityDifferentiationFibroblastMesenchymal stem cellMuscle regenerationMyogenesisPericyteSatellite cellStem cell

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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

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FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
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FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

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Area of Science:

  • Muscle stem cell biology
  • Skeletal muscle regeneration
  • Cellular heterogeneity

Background:

  • Tissue-specific stem cells are crucial for adult tissue maintenance and repair.
  • Skeletal muscle contains various mononuclear cells capable of muscle differentiation.
  • Satellite cells (SCs) are the primary stem cell population studied for muscle repair and regeneration.

Purpose of the Study:

  • To discuss diverse stem cell types contributing to skeletal muscle.
  • To focus on satellite cell heterogeneity in myogenesis and muscle regeneration.
  • To highlight methods for characterizing muscle stem cell heterogeneity.

Main Methods:

  • Review of existing literature on muscle stem cells.
  • Focus on satellite cell subpopulations and their functions.
  • Discussion of emerging techniques for stem cell characterization.

Main Results:

  • Multiple stem cell types can contribute to skeletal muscle.
  • Significant heterogeneity exists within the satellite cell pool.
  • Different subpopulations exhibit distinct behaviors in proliferation, differentiation, and self-renewal.

Conclusions:

  • Satellite cell heterogeneity is a critical factor in muscle development, repair, and aging.
  • Further characterization of muscle stem cell heterogeneity will advance understanding of muscle diseases.
  • Appreciating stem cell diversity is essential for regenerative medicine strategies.