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We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific...
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Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker...
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Targeting brain-resident cells for direct lineage-reprogramming offers new perspectives for brain repair. Here we describe a protocol of how to prepare cultures enriched for brain-resident pericytes from the adult human cerebral cortex and convert these into induced neurons by retrovirus-mediated expression of the transcription factors Sox2 and...
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Updated: Jan 20, 2026

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
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TLR8 reprograms human Treg metabolism and function

Xia Liu1, Lingyun Li1, Guangyong Peng1

  • 1Division of Infectious Diseases, Allergy and Immunology, Department of Internal Medicine, Saint Louis University School of Medicine, Saint Louis, MO 63104, USA.

Aging
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PubMed
Summary

No abstract available in PubMed .

Keywords:
TLR8Treg cells, metabolismcancer immunotherapyglycolysis

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