Related Experiment Video
Updated: Jan 20, 2026
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
[Resveratrol counteracts lipopolisaccharide-mediated oxidative stress by upregulating SOCS-1 production]
1Department of Endodontics, School of Stomatology, China Medical University. Shenyang 110002,China.
Purpose:
To investigate the protective effect and mechanism of resveratrol on oxidative stress of MC3T3-E1.
Methods:
The levels of reactive oxygen species in the cells were observed by fluorescence microscope and flow cytometry. The expression of SOCS-1 protein was detected by Western blot. SOCS-1 transient transfected cell line was established, and the levels of reactive oxygen species in transfected cells were observed by fluorescence microscopy and flow cytometry. The data were analyzed using SPSS22.0 software package.
Results:
The level of ROS in LPS group was significantly higher than that in the blank group and LPS+RES group (P<0.05). The expression level of SOCS-1 protein was increased after LPS stimulation for 30 min (P<0.05). The level of ROS in the siSOCS-1+LPS+RES group was significantly higher than that in the untransfected group (P<0.05).
Conclusions:
Resveratrol may counteract LPS-mediated oxidative stress in MC3T3E1 cells by modulating SOCS-1 protein.
Related Concept Videos
11:00Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
06:39Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
11:05Analysis of Oxidative Stress in Zebrafish Embryos
08:23Comet Assay as an Indirect Measure of Systemic Oxidative Stress
09:53Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
05:10A Spot Plate Assay for Evaluating Oxidative Stress Responses in Bacterial Strains