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Related Experiment Video

Updated: Jan 20, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

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Hepatitis C Core-Antigen Testing from Dried Blood Spots.

Mia J Biondi1,2,3, Marjolein van Tilborg4,5, David Smookler6,7

  • 1Toronto Centre for Liver Disease, Toronto General Hospital, University Health Network, University of Toronto, Toronto, ON M5G 2C4, Canada. mia.biondi@mail.mcgill.ca.

Viruses
|September 7, 2019
PubMed
Summary

Dried blood spot (DBS) testing for hepatitis C virus (HCV) antibodies and core antigen shows high accuracy across various temperatures. This method offers a viable alternative to HCV RNA testing, improving accessibility and reducing costs for HCV screening.

Keywords:
Chronic hepatitis Ccore-antigendiagnosisdried blood spotwidespread screening

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Area of Science:

  • Virology
  • Immunology
  • Diagnostic Medicine

Background:

  • Hepatitis C virus (HCV) screening requires accessible and cost-effective diagnostic methods.
  • Dried blood spot (DBS) collection offers a promising alternative for sample collection and transportation.

Purpose of the Study:

  • To evaluate the diagnostic accuracy of HCV antibody (HCV-Ab) and HCV core antigen (core-Ag) testing using DBS cards under various temperature conditions.
  • To compare the performance of DBS testing with venous and capillary blood samples.

Main Methods:

  • Blood samples from 68 chronic HCV patients were collected on DBS cards and stored for one week at temperatures ranging from -80 °C to 37 °C.
  • Eluted samples were tested for HCV-Ab and core-Ag.
  • A head-to-head comparison of venous vs. capillary samples and single vs. double DBS spots was performed.

Main Results:

  • High sensitivity for HCV-Ab detection was observed across all tested temperatures.
  • Core-Ag sensitivity remained high (91-94%) across different storage temperatures.
  • No significant difference in core-Ag sensitivity was found between venous and capillary samples, with improved sensitivity using two DBS spots.

Conclusions:

  • HCV-Ab and core-Ag testing using DBS cards demonstrates high diagnostic accuracy and stability under various temperature conditions.
  • DBS testing is a reliable alternative to HCV RNA testing, particularly in settings where accessibility and cost are considerations.
  • Further validation supports the use of DBS cards for expanded HCV screening programs.