Related Experiment Video
Updated: Jan 20, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Published on: October 2, 2018
The Effects of Caffeine and Citrus Aurantium on Performance During Repeated Maximal Anaerobic Exercise Bouts in
Cherilyn N McLester1, Paul Bailey, Emily E Bechke
1Department of Exercise Science and Sport Management, Kennesaw State University, Kennesaw, Georgia.
Abstract:
McLester, CN, Bailey, P, Bechke, EE, Williamson, CM, McLester, JR, and Kliszczewicz, B. The effects of caffeine and Citrus aurantium on performance during repeated maximal anaerobic exercise bouts in habitual caffeine users. J Strength Cond Res 35(12): 3394-3399, 2021-The combination of caffeine (CAF) and Citrus aurantium (CA) have demonstrated the potential to improve various types of performance. To date, few studies have explored this relationship in purely anaerobic-based exercise. Therefore, the purpose of this study was to examine the influence of an acute dose of CAF + CA on peak anaerobic performance and the attenuation of power over multiple anaerobic bouts and to determine any differences in perceived effort or fatigue. Ten active men (25.1 ± 3.9 years) who habitually consumed caffeine volunteered to perform repeated anaerobic bouts on a cycle ergometer on 2 separate days, consuming either a placebo or 100 mg CAF + 100 mg CA. Significance was set at p ≤ 0.05 and repeated measures analysis of variance showed no main effects for peak power p = 0.520, mean power p = 0.926, minimum power p = 0.321, total work p = 0.924, time to peak power p = 0.536, or rate of fatigue p = 0.284. There was a time effect for all variables (p ≤ 0.05) with the exception of time to peak power (p = 0.181). There were no differences in any measures of perceived effort or fatigue between conditions (p ≥ 0.05), but there were time-dependent differences observed each day (p ≤ 0.05). Overall, an acute dosage of 100 mg CAF and 100 mg CA did not elicit any differences in anaerobic performance or in perceived measures of effort and fatigue in young males who habitually consume caffeine. Therefore, caution should be used when pairing CAF and CA for the goal of improving anaerobic performance, because no clear benefit was realized in this population at this dosage.
More Related Videos
08:33Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
Published on: July 24, 2018
06:28A Chronic High-Intensity Interval Training and Diet-Induced Obesity Model to Maximize Exercise Effort and Induce Physiologic Changes in Rats
Published on: April 28, 2023
Related Concept Videos
09:11Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
08:33Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
06:28A Chronic High-Intensity Interval Training and Diet-Induced Obesity Model to Maximize Exercise Effort and Induce Physiologic Changes in Rats
07:40Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training
07:26Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Exercise and Muscle Performance
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...