Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

11.4K
This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression...
11.4K
Streamlines, Streaklines, and Pathlines01:18

Streamlines, Streaklines, and Pathlines

1.9K
A streamline represents the trajectory that is always tangent to the fluid's velocity vector at any given point. The velocity of a fluid particle is always directed along the streamline, ensuring the particle continuously follows the streamline's path. Streamlines are particularly useful for visualizing the overall direction of flow in a fluid system, and they provide an instantaneous representation of the flow's velocity field. In steady flow, where conditions do not change over...
1.9K
Input Efficiency II01:15

Input Efficiency II

227
In any production process, resources such as labor and capital must be allocated efficiently to maximize output. When multiple producers rely on the same fixed resources, the challenge is to distribute these inputs in a way that ensures no further improvements can be made without reducing another producer’s output.Efficiency in resource allocation is analyzed using isoquants, which represent different combinations of inputs that produce the same level of output. If an allocation allows at...
227
Input Efficiency I01:26

Input Efficiency I

224
Input Efficiency in Resource AllocationInput efficiency refers to the way productive resources like labor and capital are distributed across industries to maximize overall output. Unlike exchange efficiency, which deals with consumer goods allocation, input efficiency determines how resources are assigned to different production activities.Deciding How to Allocate ResourcesSince resources are limited, choices must be made about their use. Should engineers work in the automotive sector or the...
224
Bernoulli's Equation for Flow Along a Streamline01:30

Bernoulli's Equation for Flow Along a Streamline

1.4K
Bernoulli's equation relates the energy conservation in a fluid moving along a streamline. The equation applies to incompressible and inviscid fluids under steady flow. For such a flow, Newton's second law is applied to a small fluid element, which experiences forces due to pressure differences, gravity, and velocity variations. The force balance leads to the following form of Bernoulli's equation:
1.4K
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

20.4K
This protocol is a cost effective alternative for efficient parallel clarification and plasmid DNA purification from E. coli cultures. The AcroPrep Advance process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter...
20.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MICU1 is the nexus for Ca<sub>V</sub>3.3 regulation of mitochondrial calcium, redox balance and chondrocyte viability.

International journal of biological macromolecules·2025
Same author

Pyrifluquinazon baseline susceptibility and inhibition of Tomato chlorosis virus transmission by Bemisia tabaci.

Pest management science·2023
Same author

Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification.

Analytica chimica acta·2020
Same author

Optimization of fungi co-fermentation for improving anthraquinone contents and antioxidant activity using artificial neural networks.

Food chemistry·2020
Same author

Counter-current fractionation-assisted bioassay-guided separation of active composition from the edible medicinal insect Blaps rynchopetera Fairmaire.

Journal of chromatography. A·2019
Same author

Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.

Microbial pathogenesis·2018

Related Experiment Video

Updated: Jan 20, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

11.4K

Streamlined Low-Input Transcriptomics through EASY-RNAseq.

Yiwen Zhou1, Hao Xu1, Haiyang Wu2

  • 1Biomedical Analysis Center, Army Medical University, Chongqing, China.

Journal of Molecular Biology
|September 7, 2019
PubMed
Summary
This summary is machine-generated.

We developed EASY RNAseq, a streamlined protocol for sensitive transcriptome profiling from low-input samples. This method accurately analyzes small cell populations, detecting 70% of protein-coding genes in single human embryos.

Keywords:
RNAseqT cellsembryolow inputmRNA

More Related Videos

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

20.4K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.7K

Related Experiment Videos

Last Updated: Jan 20, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

11.4K
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

20.4K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.7K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • High-throughput sequencing is crucial for transcriptome profiling in biological research.
  • Accurate transcriptome profiling of small cell populations is challenging due to low gene detection sensitivity and complex experimental procedures.

Purpose of the Study:

  • To introduce a streamlined RNA sequencing (RNAseq) protocol, EASY RNAseq, designed for sensitive transcriptome assessment from minimal input materials.
  • To overcome the limitations of existing methods in profiling small cell populations.

Main Methods:

  • Development of a streamlined RNAseq protocol (EASY RNAseq).
  • Application of EASY RNAseq to single-human embryos at the 8-cell stage.
  • Comparative analysis of gene detection sensitivity and expression distribution patterns with other common methods.

Main Results:

  • EASY RNAseq demonstrates technical robustness for sequencing small cell pools.
  • The protocol recovers information on a larger number of genes compared to other methods.
  • EASY RNAseq exhibits a more even gene expression distribution pattern.
  • Application to single human embryos detected 70% of annotated protein-coding genes.

Conclusions:

  • EASY RNAseq provides a sensitive and robust method for transcriptome profiling of low-input samples.
  • This workflow is effective for interrogating rare cell populations.
  • The protocol enhances gene detection sensitivity and data quality for small-scale biological samples.