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Updated: Dec 29, 2025
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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification.
Yaqian Liu1, Xiong Zhang1, Min Liu1
1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, 430072, Hubei Province, China.
Analytica Chimica Acta
|February 8, 2020
Summary
Quantifying circular RNA (circRNA) is crucial for understanding its role in diseases. A new method, reverse transcription-rolling circle amplification (RT-RCA), offers a fast and selective way to detect circRNA in biological samples.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Circular RNA (circRNA) represents a novel class of endogenous non-coding RNA with conserved sequences.
- circRNA plays a role in various biological processes.
- Quantifying circRNA is essential for understanding its regulatory circuits and disease associations.
Purpose of the Study:
- To develop a method for selective amplification and quantification of target circRNA.
- To introduce a novel assay for rapid and accurate circRNA detection.
Main Methods:
- A reverse transcription mediated rolling circle amplification (RT-RCA) process was developed.
- The assay utilizes selective amplification of target circRNA.
Main Results:
- The RT-RCA assay demonstrated high selectivity for circRNA identification.
- The method allows for circRNA detection within a few hours.
Conclusions:
- RT-RCA is a promising technique for circRNA detection.
- This method can aid in detailed investigation of circRNA's role in cellular functions and human diseases.
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