Stoichiometric control on riparian wetland carbon and nutrient dynamics under different land uses

Riparian wetland provides important ecosystem function, such as water filtration and nutrient retention. When land use change in upland from native forest to sugarcane cultivation have important impacts on carbon (C) and nutrient availability in downstream wetland systems. Here, we examined concentrations and stoichiometry of C and nutrients in total, labile, biomass pools in upland soil, riparian wetland and sediment along two distinct transects (sugarcane versus forest). Sugarcane cultivation significantly reduced total C, nitrogen (N), labile C and N in riparian soils by 69%, 62%, 33% and 45%, respectively, but significantly increased NO₃^--N and δ¹⁵N by 99% and 56% in riparian areas. The presence of native forest resulted in significantly higher NH₄⁺-N concentrations in downstream wetlands. Concentrations of microbial biomass C and N were generally lower, but the abundance of genes associated with nitrifiers (ammonia oxidizing bacteria and archaea) was higher in the sugarcane transect than in the forest transect. These significantly differences between two transects could be attributed to different organic inputs and biogeochemical processes associated with the different vegetation types and management practices in the upland systems. Difference in δ¹³C signature from the two transects further confirmed the significant influence of vegetation type on downstream wetlands. Sugarcane cultivation led to a consistent stoichiometric shift in both resource and microbial biomass towards lower C:P and N:P ratios across upland soils, wetlands and sediment, compared with the forest transect. The average total and microbial biomass C:N:P ratios in soil under sugarcane were 136:9:1 and 180:33:1, respectively. The average total and microbial biomass C:N:P ratios in soil under forest were 410:22:1 and 594:76:1, respectively. It is concluded that since microbial demand of C and nutrients is driven by the stoichiometry of the biomass, which is regulated by the resource stoichiometry, a change of resource induced by upland land use change leads to a shift in the stoichiometry of microbial biomass C, N and P.