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Updated: Jan 20, 2026

Scalable Syntheses of Graphene Oxide and Reduced Graphene Oxide using Cascade Design Oxidation and Highly Basic Reduction Reactions
Published on: July 3, 2025
Ribonuclease H Enzyme Activity Detection Based on Hybridization Chain Reaction Amplification and Graphene Oxide
Cun Liu1, Ying Tang1, Yao Chen1
1Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, P. R. China.
Abstract:
Ribonuclease H (RNase H) plays key roles in HIV virus replication process and can be used as an important target for anti-AIDS drugs screening. Therefore, we constructed a fluorescent nanosensor based on signal amplification of hybridization chain reaction (HCR) and fluorescence quenching of graphene oxide (GO) nanosheets. The nanosensor provided highly sensitive and selective platform for RNase H activity detection. This method also exhibited a good linear relationship in the range of 0.001-5 U/mL, and the detection limit was 0.0007 U/mL. Our results suggested that the developed system is a promising platform for monitoring the RNase H activity, showing great potential in the biomedical studies and drugs screening.
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