Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transplantation of Cells Directly into the Kidney of Adult Zebrafish06:04

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

15.5K
Cell transplantation is an essential technique for studying tissue regeneration and for developing cell-based therapies of disease. We demonstrate here a microsurgical technique that permits the transplantation of genetically labeled cells directly into the kidney of adult zebrafish...
15.5K
Understanding the Self01:28

Understanding the Self

247
The self is a central aspect of human identity, encompassing an individual’s beliefs, emotions, perceptions, and experiences. It is a cognitive and psychological construct that enables individuals to interpret their traits and behaviors, influencing how they perceive themselves and interact with the world. While personality consists of stable and enduring characteristics, the self is shaped by self-perception and social experiences. This distinction highlights the dynamic nature of the...
247
Generating Kidney Organoids in Suspension from Induced Pluripotent Stem Cells07:22

Generating Kidney Organoids in Suspension from Induced Pluripotent Stem Cells

3.6K
This protocol presents a comprehensive and efficient method for producing kidney organoids from induced pluripotent stem cells (iPSCs) using suspension culture conditions. The primary emphasis of this study lies in the determination of the initial cell density and the WNT agonist concentration, thereby benefiting investigators interested in kidney organoid...
3.6K
Kidney Structure01:45

Kidney Structure

74.9K
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries.
74.9K
Reliable and High Efficiency Extraction of Kidney Immune Cells09:15

Reliable and High Efficiency Extraction of Kidney Immune Cells

10.9K
Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this...
10.9K
Isolation of Double Negative αβ T Cells from the Kidney06:56

Isolation of Double Negative αβ T Cells from the Kidney

12.8K
DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Validation of a Simplified Martin-Hopkins LDL-C Equation Using Machine Learning.

JAMA cardiology·2026
Same author

Psychological Factors Applicable to App Development for Overcoming Problematic Smartphone Use: A Structural Equation Modeling Approach.

Psychiatry investigation·2026
Same author

A Co-opted Developmental Gene Regulatory Program in Endothelial Progenitors Promotes Tumor Angiogenic Phenotypes.

Cancer research·2026
Same author

Generation of spermatid-like cells from testicular biopsies obtained from prepubertal boys.

F&S science·2026
Same author

Dissecting the coordinated progression of cell states in spatial transcriptomics with CoPro.

bioRxiv : the preprint server for biology·2026
Same author

Analysis of microsatellite instability intensity in single-cell resolution with scMnT reveals tumor heterogeneity in colorectal cancer.

Briefings in bioinformatics·2026

Related Experiment Video

Updated: Jan 19, 2026

Transplantation of Cells Directly into the Kidney of Adult Zebrafish
06:04

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Published on: May 18, 2011

15.5K

Understanding the kidney one cell at a time.

Jihwan Park1, Chang Linda Liu1, Junhyong Kim2

  • 1Renal, Electrolyte, and Hypertension Division, Department of Medicine, Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Kidney International
|September 8, 2019
PubMed
Summary

New cellular measurement technologies allow genome-wide analysis of individual kidney cells. This advances our understanding of kidney development, homeostasis, and disease beyond traditional methods.

Keywords:
RNA-seqepigenomekidney diseasesingle cell

More Related Videos

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids
07:22

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

Published on: September 1, 2023

3.6K
Reliable and High Efficiency Extraction of Kidney Immune Cells
09:15

Reliable and High Efficiency Extraction of Kidney Immune Cells

Published on: August 19, 2016

10.9K

Related Experiment Videos

Last Updated: Jan 19, 2026

Transplantation of Cells Directly into the Kidney of Adult Zebrafish
06:04

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Published on: May 18, 2011

15.5K
Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids
07:22

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

Published on: September 1, 2023

3.6K
Reliable and High Efficiency Extraction of Kidney Immune Cells
09:15

Reliable and High Efficiency Extraction of Kidney Immune Cells

Published on: August 19, 2016

10.9K

Area of Science:

  • Genomics
  • Cellular Biology
  • Biotechnology

Background:

  • Previous renal studies analyzed averaged tissue outputs.
  • Morphotype-based descriptions limited organ and disease understanding.

Purpose of the Study:

  • To introduce revolutionary cellular measurement technology for renal studies.
  • To enable genome-wide analysis of individual cells.
  • To shift from morphotype-based to genomic definitions in nephrology.

Main Methods:

  • Accurate monitoring of genome-wide gene expression.
  • Analysis of gene regulation, function, and cellular history.
  • Simultaneous study of cellular interactions in thousands of individual cells.

Main Results:

  • Enabled precise measurement of individual cell genomics.
  • Facilitated comprehensive analysis of cellular processes.
  • Provided a foundation for unbiased genomic definitions of kidney states.

Conclusions:

  • Revolutionary cellular measurement technology is transforming renal research.
  • Genomic insights from single-cell analysis improve understanding of kidney development, homeostasis, and disease.