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The cloning of the rorB gene of Escherichia coli
P G Debenham1, M B Webb, J Law
1Division of Cell and Molecular Biology, M.R.C. Radiobiology Unit, Didcot, Oxon, UK.
Molecular & General Genetics : MGG
|December 1, 1988
Abstract:
A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex.