Related Experiment Video
Updated: Jan 19, 2026
Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
Published on: December 5, 2025
Genomic Mushroom Hunting Decrypts Coprinoferrin, A Siderophore Secondary Metabolite Vital to Fungal Cell Development
Yuta Tsunematsu1, Jun Takanishi1, Shihori Asai1
1Department of Pharmaceutical Sciences , University of Shizuoka , Shizuoka 422-8526 , Japan.
Abstract:
LaeA is a positive global regulator of secondary metabolism in Ascomycetes, but its role in Basidiomycetes, including medicinal mushrooms, remains uncharacterized. Here, knockout of laeA in the model mushroom Coprinopsis cinerea unexpectedly upregulated the biosynthesis of a novel siderophore, coprinoferrin. Furthermore, knockout of the nonribosomal peptide synthetase-encoding cpf1 responsible for coprinoferrin biosynthesis resulted in growth defect and loss of fruiting body formation, indicating the complex role that this natural product plays in fungal cell development.
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ExpandTo begin, collect your allocated culture plates and then remove the Parafilm and lid from one of them.
Place the uncovered plate under a dissecting microscope and then observe the individual communities within each quadrant of the plate.
As the colonies are identified, fill out the observed colony properties in the appropriate column in the table. Click Here to download Table 1
Then, repeat the observations for each of the other two bacterial...
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