Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice07:00

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

11.0K
Chronic ocular hypertension is induced by applying a circumlimbal suture in rats and mice, leading to functional and structural deterioration of the retinal ganglion cells consistent with...
11.0K
In Vivo Imaging of Retinal Microglia in a Mouse Model of Glaucoma04:55

In Vivo Imaging of Retinal Microglia in a Mouse Model of Glaucoma

613
Source: Bosco, A., et al. In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma. J. Vis. Exp. (2015) This video demonstrates in vivo imaging of retinal microglia in a mouse model of glaucoma. A transgenic mouse with fluorescent protein-expressing retinal microglia is positioned under an ophthalmoscope. Infrared illumination is used to locate the optic nerve head (ONH), and the focal...
613
Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling20:36

Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling

9.1K
Charles Taylor and John Marshall explain the utility of mathematical modeling for evaluating the effectiveness of population replacement strategy. Insight is given into how computational models can provide information on the population dynamics of mosquitoes and the spread of transposable elements through A. gambiae subspecies. The ethical considerations of releasing genetically modified mosquitoes into the wild are...
9.1K
Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation08:30

Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation

13.6K
Glaucoma is characterized by damage to retinal ganglion cells. Inducing glaucoma in animal models can provide insight into the study of this disease. Here, we outline a procedure that induces loss of RGCs in an in vivo rat model and demonstrates the preparation of whole-mount retinas for analysis.
13.6K
A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice09:26

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

20.5K
Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous...
20.5K
Ultrasound Cyclo Plasty in Eyes with Glaucoma05:05

Ultrasound Cyclo Plasty in Eyes with Glaucoma

12.7K
Glaucoma is a chronic disease with progressive degeneration of optic nerve fibers resulting in decreased visual field. Elevated intraocular pressure is considered the most important and the only treatable risk factor. This manuscript describes a simple, surgeon-friendly, non-incisional technique, named Ultrasound Cyclo Plasty, for reducing intraocular pressure in glaucoma...
12.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging role of stem cells and tissue engineering in oculoplastic surgery.

Indian journal of ophthalmology·2026
Same author

Integrating yoga as a holistic intervention in male infertility management: improved sperm parameters and stress reduction: a case report.

Journal of medical case reports·2026
Same author

Gillespie syndrome caused by a novel <i>ITPR1</i> gene variant: a phenotype-genotype correlation.

Ophthalmic genetics·2026
Same author

Biomarkers for Endometrial Receptivity: Implications for Infertility, Implantation Failure, and Advances in Diagnosis and Treatment.

International journal of molecular sciences·2026
Same author

The role of epigenetic signatures in type 2 diabetes: therapeutic advances and lifestyle interventions.

Acta diabetologica·2026
Same author

Lipemia retinalis: a case study of a rare finding in pediatric retina secondary to diabetes.

Canadian journal of ophthalmology. Journal canadien d'ophtalmologie·2026

Related Experiment Video

Updated: Jan 19, 2026

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice
07:00

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Published on: October 5, 2018

11.0K

A Novel Mathematical Model of Glaucoma Pathogenesis.

Muneeb A Faiq1, Talvir Sidhu1, Rayees A Sofi2

  • 1Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Journal of Current Glaucoma Practice
|September 10, 2019
PubMed
Summary

This study introduces the first mathematical model for glaucoma pathogenesis, identifying microglia activation as an early event. This model aids in understanding disease progression and developing diagnostic and therapeutic strategies.

Keywords:
GlaucomaGlial activationInflammationMathematical modelingNeuron–glia interactionRetinal ganglion cell

More Related Videos

Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling
20:36

Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling

Published on: July 4, 2007

9.1K
Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation
08:30

Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation

Published on: March 12, 2016

13.6K

Related Experiment Videos

Last Updated: Jan 19, 2026

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice
07:00

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Published on: October 5, 2018

11.0K
Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling
20:36

Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling

Published on: July 4, 2007

9.1K
Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation
08:30

Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation

Published on: March 12, 2016

13.6K

Area of Science:

  • Ophthalmology
  • Computational Biology
  • Systems Biology

Background:

  • Glaucoma pathogenesis is complex, involving numerous molecular and cellular factors interacting with environmental influences.
  • Conventional experimental methods struggle to unravel these intricate interactions and predict disease progression.
  • Understanding these complex interactions is crucial for effective glaucoma management.

Purpose of the Study:

  • To develop a novel mathematical model for analyzing glaucoma pathogenesis.
  • To identify key cellular events and their interactions in disease development.
  • To provide insights into early indicators and potential therapeutic targets for glaucoma.

Main Methods:

  • A system-level analysis using mathematical modeling and rate equations was performed.
  • Twenty-two key events in glaucoma pathogenesis were identified and analyzed.
  • Sensitivity coefficients for retinal ganglion cells and cellular stress were calculated and time-course analyses were conducted.

Main Results:

  • Microglia activation was identified as one of the earliest events in glaucoma pathogenesis.
  • The mathematical model provides valuable insights into the sensitivity coefficients of cellular events.
  • The study highlights the role of glia-mediated pathology in glaucoma.

Conclusions:

  • This novel mathematical model offers a new approach to understanding glaucoma pathogenesis.
  • The findings can guide experimental design, diagnostics, and therapeutic target identification.
  • The model emphasizes the significance of early events like microglia activation and glia-mediated processes.